This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Velázquez, F.
Right arrow Articles by de Lorenzo, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Velázquez, F.
Right arrow Articles by de Lorenzo, V.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2004, p. 8267-8275, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8267-8275.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Evidence that Catabolites of the Entner-Doudoroff Pathway Signal C Source Repression of the {sigma}54 Pu Promoter of Pseudomonas putida

Francisco Velázquez,1 Ilaria di Bartolo,2 and Víctor de Lorenzo1*

Centro Nacional de Biotecnología CSIC, Campus de Cantoblanco, Madrid, Spain,1 Dipartimento di Biologia, Università di Roma Tre, Rome, Italy2

Received 25 July 2004/ Accepted 13 September 2004

Glucose and other C sources exert an atypical form of catabolic repression on the {sigma}54-dependent promoter Pu, which drives transcription of an operon for m-xylene degradation encoded by the TOL plasmid pWW0 in Pseudomonas putida. We have used a genetic approach to identify the catabolite(s) shared by all known repressive C sources that appears to act as the intracellular signal that triggers downregulation of Pu. To this end, we reconstructed from genomic data the pathways for metabolism of repressor (glucose, gluconate) and nonrepressor (fructose) C sources. Since P. putida lacks fructose-6-phosphate kinase, glucose and gluconate appear to be metabolized exclusively by the Entner-Doudoroff (ED) pathway, while fructose can be channeled through the Embden-Meyerhof (EM) route. An insertion in the gene fda (encoding fructose-1,6-bisphosphatase) that forces fructose metabolism to be routed exclusively to the ED pathway makes this sugar inhibitory for Pu. On the contrary, a crc mutation known to stimulate expression of the ED enzymes causes the promoter to be less sensitive to glucose. Interrupting the ED pathway by knocking out eda (encoding 2-dehydro-3-deoxyphosphogluconate aldolase) exacerbates the inhibitory effect of glucose in Pu. These observations pinpoint the key catabolites of the ED route, 6-phosphogluconate and/or 2-dehydro-3-deoxyphosphogluconate, as the intermediates that signal Pu repression. This notion is strengthened by the observation that 2-ketogluconate, which enters the ED pathway by conversion into these compounds, is a strong repressor of the Pu promoter.

* Corresponding author. Mailing address: Centro Nacional de Biotecnología del CSIC, 28049 Madrid, Spain. Phone: 34 91 585 4536. Fax: 43 91 585 4506. E-mail: vdlorenzo{at}cnb.uam.es.

Journal of Bacteriology, December 2004, p. 8267-8275, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8267-8275.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Daddaoua, A., Krell, T., Ramos, J.-L. (2009). Regulation of Glucose Metabolism in Pseudomonas: THE PHOSPHORYLATIVE BRANCH AND ENTNER-DOUDOROFF ENZYMES ARE REGULATED BY A REPRESSOR CONTAINING A SUGAR ISOMERASE DOMAIN. J. Biol. Chem. 284: 21360-21368 [Abstract] [Full Text]  
  • Kim, J., Yeom, J., Jeon, C. O., Park, W. (2009). Intracellular 2-keto-3-deoxy-6-phosphogluconate is the signal for carbon catabolite repression of phenylacetic acid metabolism in Pseudomonas putida KT2440. Microbiology 155: 2420-2428 [Abstract] [Full Text]  
  • Pfluger, K., de Lorenzo, V. (2008). Evidence of In Vivo Cross Talk between the Nitrogen-Related and Fructose-Related Branches of the Carbohydrate Phosphotransferase System of Pseudomonas putida. J. Bacteriol. 190: 3374-3380 [Abstract] [Full Text]  
  • del Castillo, T., Duque, E., Ramos, J. L. (2008). A Set of Activators and Repressors Control Peripheral Glucose Pathways in Pseudomonas putida To Yield a Common Central Intermediate. J. Bacteriol. 190: 2331-2339 [Abstract] [Full Text]  
  • del Castillo, T., Ramos, J. L. (2007). Simultaneous Catabolite Repression between Glucose and Toluene Metabolism in Pseudomonas putida Is Channeled through Different Signaling Pathways. J. Bacteriol. 189: 6602-6610 [Abstract] [Full Text]  
  • del Castillo, T., Ramos, J. L., Rodriguez-Herva, J. J., Fuhrer, T., Sauer, U., Duque, E. (2007). Convergent Peripheral Pathways Catalyze Initial Glucose Catabolism in Pseudomonas putida: Genomic and Flux Analysis. J. Bacteriol. 189: 5142-5152 [Abstract] [Full Text]  
  • Pfluger, K., de Lorenzo, V. (2007). Growth-dependent Phosphorylation of the PtsN (EIINtr) Protein of Pseudomonas putida. J. Biol. Chem. 282: 18206-18211 [Abstract] [Full Text]  
  • Velazquez, F., Pfluger, K., Cases, I., De Eugenio, L. I., de Lorenzo, V. (2007). The Phosphotransferase System Formed by PtsP, PtsO, and PtsN Proteins Controls Production of Polyhydroxyalkanoates in Pseudomonas putida. J. Bacteriol. 189: 4529-4533 [Abstract] [Full Text]  
  • Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
  • Aranda-Olmedo, I., Marin, P., Ramos, J. L., Marques, S. (2006). Role of the ptsN Gene Product in Catabolite Repression of the Pseudomonas putida TOL Toluene Degradation Pathway in Chemostat Cultures{triangledown}. Appl. Environ. Microbiol. 72: 7418-7421 [Abstract] [Full Text]  
  • Aranda-Olmedo, I., Ramos, J. L., Marques, S. (2005). Integration of Signals through Crc and PtsN in Catabolite Repression of Pseudomonas putida TOL Plasmid pWW0. Appl. Environ. Microbiol. 71: 4191-4198 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»