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Journal of Bacteriology, December 2004, p. 8317-8325, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8317-8325.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Negative Regulation of DNA Repair Gene (ung) Expression by the CpxR/CpxA Two-Component System in Escherichia coli K-12 and Induction of Mutations by Increased Expression of CpxR

Hiroshi Ogasawara,1 Jun Teramoto,1 Kiyo Hirao,1 Kaneyoshi Yamamoto,1 Akira Ishihama,2 and Ryutaro Utsumi1*

Department of Bioscience and Biotechnology, Graduate School of Agriculture of Kinki University, Nakamachi, Nara,1 Division of Molecular Biology, Nippon Institute for Biological Science, Ome, Tokyo, Japan2

Received 21 July 2004/ Accepted 10 September 2004

In Escherichia coli K-12 overexpressing CpxR, transcription of the ung gene for uracil-DNA glycosylase was repressed, ultimately leading to the induction of mutation. Gel shift, DNase I footprinting, and in vitro transcription assays all indicated negative regulation of ung transcription by phosphorylated CpxR. Based on the accumulated results, we conclude that ung gene expression is negatively regulated by the two-component system of CpxR/CpxA signal transduction.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, Graduate School of Agriculture of Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan. Phone: 81-742-43-7306. Fax: 81-742-43-1445. E-mail: utsumi{at}nara.kindai.ac.jp.

Journal of Bacteriology, December 2004, p. 8317-8325, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8317-8325.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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