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Journal of Bacteriology, December 2004, p. 8499-8507, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8499-8507.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

RpoS-Regulated Genes of Escherichia coli Identified by Random lacZ Fusion Mutagenesis

Somalinga R. V. Vijayakumar ,{dagger},{ddagger} Mark G. Kirchhof,{dagger},§ Cheryl L. Patten, and Herb E. Schellhorn*

Department of Biology, McMaster University, Hamilton, Ontario, Canada

Received 25 June 2004/ Accepted 10 September 2004

RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differential expression of an operon-lacZ fusion bank in rpoS mutant and wild-type backgrounds. Forty-eight independent gene fusions were identified, including several in well-characterized RpoS-regulated genes, such as osmY, katE, and otsA. Many of the other fusions mapped to genes of unknown function or to genes that were not previously known to be under RpoS control. Based on the homology to other known bacterial genes, some of the RpoS-regulated genes of unknown functions are likely important in nutrient scavenging.

* Corresponding author. Mailing address: Department of Biology, McMaster University, 1280 Main St. West, Hamilton, Ontario, Canada, L8S 4K1. Phone: (905) 525-9140 ext. 27316. Fax: (905) 522-6066. E-mail: schell{at}mcmaster.ca.

{dagger} Contributed equally to this paper.

{ddagger} Present address: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada, N6A 5C1.

§ Present address: Robarts Research Institute, University of Western Ontario, London, Ontario, Canada, N6A 5K8.

Journal of Bacteriology, December 2004, p. 8499-8507, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8499-8507.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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