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Journal of Bacteriology, December 2004, p. 8537-8541, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8537-8541.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Influence of L-Leucine and L-Alanine on Lrp Regulation of foo, Coding for F1651, a Pap Homologue

Frédéric Berthiaume,1 Cécile Crost,1,2 Vincent Labrie,1 Christine Martin,2 Elaine B. Newman,3 and Josée Harel1*

Département de Pathologie et Microbiologie, Université de Montréal, St-Hyacinthe,1 Department of Biology, Concordia University, Montréal, Quebec, Canada,3 Laboratoire de Microbiologie, Institut National de la Recherche Agronomique, St-Genès-Champanelle, France2

Received 8 January 2004/ Accepted 6 September 2004

The foo operon encodes F1651 fimbriae that belong to the P-regulatory family and are synthesized by septicemic Escherichia coli. Using an Lrp-deficient host and the lrp gene cloned under the arabinose pBAD promoter, we demonstrated that foo was transcribed proportionally to the amount of Lrp synthesized. L-Leucine and L-alanine decreased drastically the steady-state transcription of foo and modified phase variation, independently of the presence of FooI. Specific mutations in the C-terminal region of Lrp reduced or abolished the repressive effect of these amino acids, indicating that they modulate F1651 by affecting Lrp.

* Corresponding author. Mailing address: Département de Pathologie et Microbiologie, Université de Montréal, C.P. 5000, St-Hyacinthe, QC, Canada J2S 7C6. Phone: (450) 773-8521, ext. 8233. Fax: (450) 778-8108. E-mail: josee.harel{at}umontreal.ca.

Journal of Bacteriology, December 2004, p. 8537-8541, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8537-8541.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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