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Journal of Bacteriology, February 2004, p. 692-698, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.692-698.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Quorum Sensing-Controlled Biofilm Development in Serratia liquefaciens MG1

Maurizio Labbate,1,2 Shu Yeong Queck,1,2 Kai Shyang Koh,1,2 Scott A. Rice,1,2 Michael Givskov,3 and Staffan Kjelleberg1,2*

School of Biotechnology and Biomolecular Sciences,1 Centre for Marine Biofouling and Bio-Innovation, University of New South Wales, Sydney, New South Wales, Australia,2 Section of Molecular Microbiology, BioCentrum-DTU, The Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark3

Received 27 May 2003/ Accepted 27 October 2003

Serratia liquefaciens MG1 contains an N-acylhomoserine lactone-mediated quorum-sensing system that is known to regulate swarming motility colonization. In this study, we describe for S. liquefaciens MG1 the development of a novel biofilm consisting of cell aggregates and differentiated cell types, such as cell chains and long filamentous cells. Furthermore, quorum sensing is shown to be crucial for normal biofilm development and for elaborate differentiation. A mutant of S. liquefaciens MG1 that was incapable of synthesizing extracellular signal formed a thin and nonmature biofilm lacking cell aggregates and differentiated cell chains. Signal-based complementation of this mutant resulted in a biofilm with the wild-type architecture. Two quorum-sensing-regulated genes (bsmA and bsmB) involved in biofilm development were identified, and we propose that these genes are engaged in fine-tuning the formation of cell aggregates at a specific point in biofilm development.

* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia 2052. Phone: 61-2-9385 2102. Fax: 61-2-9385 1779. E-mail: s.kjelleberg{at}unsw.edu.au.

Journal of Bacteriology, February 2004, p. 692-698, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.692-698.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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