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Journal of Bacteriology, February 2004, p. 811-817, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.811-817.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The trans-Acting Protein Interacting with the DNA Motif Proximal to the Transcriptional Start Site of Plant L-Asparaginase Is Bacterial Sarcosine Oxidase

William T. Jones,^* Taha Al-Samarrai, Janice M. Reeves, Gordon B. Ryan, Christopher A. Kirk, Eva Vincze, Dawn Harvey, Marie McCambridge, David Greenwood, and Paul H. S. Reynolds

Horticultural Research Institute of New Zealand, Palmerston North, New Zealand

Received 19 June 2003/ Accepted 29 October 2003

A trans-acting protein interacting with a specific sequence motif proximal to the transcriptional start site of the L-asparaginase promoter has been observed previously (E. Vincze, J. M. Reeves, E. Lamping, K. J. F. Farnden, and P. H. S. Reynolds, Plant Mol. Biol. 26:303-311, 1994). Gel retardation experiments in which protein extracts of Mesorhizobium loti and developing nodules were used suggested a bacterial origin for the repressor binding protein (rep2037). Nodulation tests were performed by using different Fix^- Tn5 mutants of M. loti. Analyses of these mutants revealed a correlation between the presence of Mesorhizobium in the nodule-like structures and the ability of nodule protein extracts to bind the repressor binding domain (RBD). Through the use of mutated RBD sequences, the RBD sequence was identified as CTAAAAT. The repressor protein was isolated from M. loti NZP2037 by multiple chromatographic procedures and affinity separation by using concatemers of RBD attached to magnetic beads. Sequencing of the recovered protein resulted in identification of the repressor protein as the sarcosine oxidase subunit. This was confirmed by expression of the gene encoding the M. loti subunit of sarcosine oxidase in Escherichia coli. When the expressed peptide was bound to RBD, the gel retardation result was identical to the result obtained with rep2037 from M. loti strain NZP2037.

Present address: Danish Institute of Agricultural Science, Research Centre Flakkebjerg, Slagelse, Denmark.

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