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Journal of Bacteriology, February 2004, p. 818-828, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.818-828.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Regulation of the Tryptophan Biosynthetic Genes in Bacillus halodurans: Common Elements but Different Strategies than Those Used by Bacillus subtilis

Reka Szigeti,{dagger} Mirela Milescu, and Paul Gollnick*

Department of Biological Sciences, State University of New York, Buffalo, New York 14260

Received 27 August 2003/ Accepted 23 October 2003

In Bacillus subtilis, an RNA binding protein called TRAP regulates both transcription and translation of the tryptophan biosynthetic genes. Bacillus halodurans is an alkaliphilic Bacillus species that grows at high pHs. Previous studies of this bacterium have focused on mechanisms of adaptation for growth in alkaline environments. We have characterized the regulation of the tryptophan biosynthetic genes in B. halodurans and compared it to that in B. subtilis. B. halodurans encodes a TRAP protein with 71% sequence identity to the B. subtilis protein. Expression of anthranilate synthetase, the first enzyme in the pathway to tryptophan, is regulated significantly less in B. halodurans than in B. subtilis. Examination of the control of the B. halodurans trpEDCFBA operon both in vivo and in vitro shows that only transcription is regulated, whereas in B. subtilis both transcription of the operon and translation of trpE are controlled. The attenuation mechanism that controls transcription in B. halodurans is similar to that in B. subtilis, but there are some differences in the predicted RNA secondary structures in the B. halodurans trp leader region, including the presence of a potential anti-antiterminator structure. Translation of trpG, which is within the folate operon in both bacilli, is regulated similarly in the two species.

* Corresponding author. Mailing address: Department of Biological Sciences, State University of New York, Buffalo, NY 14260. Phone: (716) 645-2363, ext. 189. Fax: (716) 645-2975. E-mail: gollnick{at}acsu.buffalo.edu.

{dagger} Present address: Department of Dermatology, Medical University of Pecs, Kodaly u. 20, Hungary.

Journal of Bacteriology, February 2004, p. 818-828, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.818-828.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McGraw, A. P., Mokdad, A., Major, F., Bevilacqua, P. C., Babitzke, P. (2009). Molecular basis of TRAP-5'SL RNA interaction in the Bacillus subtilis trp operon transcription attenuation mechanism. RNA 15: 55-66 [Abstract] [Full Text]  
  • McGraw, A. P., Bevilacqua, P. C., Babitzke, P. (2007). TRAP-5' stem loop interaction increases the efficiency of transcription termination in the Bacillus subtilis trpEDCFBA operon leader region. RNA 13: 2020-2033 [Abstract] [Full Text]  


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