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Journal of Bacteriology, February 2004, p. 885-888, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.885-888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Motility of Urease-Deficient Derivatives of Helicobacter pylori

Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110

Received 30 July 2003/ Accepted 30 October 2003

Early studies of a ureB mutant derivative of Helicobacter pylori had suggested that urease is needed for motility and that urease action helps energize flagellar rotation. Here we report experiments showing that motility is unaffected by deletion of ureA and ureB (urease genes) or by inactivation of ureB alone, especially if H. pylori strains used as recipients for transformation with mutant alleles are preselected for motility. This result was obtained with the strain used in the early studies (CPY3401) and also with 15 other strains, 3 of which can colonize mice. We conclude that urease is not needed for H. pylori motility.

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