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Journal of Bacteriology, February 2004, p. 1097-1105, Vol. 186, No. 4
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.4.1097-1105.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Differential Expression of Two Paralogous Genes of Bacillus subtilis Encoding Single-Stranded DNA Binding Protein

Cordula Lindner,{dagger},{ddagger} Reindert Nijland,{dagger}, Mariska van Hartskamp,§ Sierd Bron, Leendert W. Hamoen, and Oscar P. Kuipers*

Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, NL-9751 NN Haren, The Netherlands

Received 2 July 2003/ Accepted 31 October 2003

The Bacillus subtilis genome comprises two paralogous single-stranded DNA binding protein (SSB) genes, ssb and ywpH, which show distinct expression patterns. The main ssb gene is strongly expressed during exponential growth and is coregulated with genes encoding the ribosomal proteins S6 and S18. The gene organization rpsF-ssb-rpsR as observed in B. subtilis is found in many gram-positive as well as some gram-negative bacteria, but not in Escherichia coli. The ssb gene is essential for cell viability, and like other SSBs its expression is elevated during SOS response. In contrast, the paralogous ywpH gene is transcribed from its own promoter at the onset of stationary phase in minimal medium only. Its expression is ComK dependent and its gene product is required for optimal natural transformation.

* Corresponding author. Mailing address: Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, Kerklaan 30, NL-9751 NN Haren, The Netherlands. Phone: 31 50 3632093. Fax: 31 50 3632348. E-mail: o.p.kuipers{at}biol.rug.nl.

{dagger} C.L. and R.N. contributed equally to this article.

{ddagger} Present address: FCDF Corporate Research, 7418 BA Deventer, The Netherlands.

§ Present address: PURAC Biochem, 4200 AA Gorinchem, The Netherlands.

Present address: Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.

Journal of Bacteriology, February 2004, p. 1097-1105, Vol. 186, No. 4
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.4.1097-1105.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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