This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Isticato, R.
Right arrow Articles by Ricca, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Isticato, R.
Right arrow Articles by Ricca, E.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2004, p. 1129-1135, Vol. 186, No. 4
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.4.1129-1135.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Assembly of Multiple CotC Forms into the Bacillus subtilis Spore Coat

Rachele Isticato,1 Giovanni Esposito,1 Rita Zilhão,2,3 Sofia Nolasco,3,{dagger} Giuseppina Cangiano,1 Maurilio De Felice,1 Adriano O. Henriques,2 and Ezio Ricca1*

Dipartimento di Fisiologia Generale ed Ambientale, Università Federico II, Naples, Italy,1 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2781-901 Oeiras,2 Departmento de Biologia Vegetal, Universidade de Lisboa, Lisbon, Portugal3

Received 2 July 2003/ Accepted 12 September 2003

We report evidence that the CotC polypeptide, a previously identified component of the Bacillus subtilis spore coat, is assembled into at least four distinct forms. Two of these, having molecular masses of 12 and 21 kDa, appeared 8 h after the onset of sporulation and were probably assembled on the forming spore immediately after their synthesis, since no accumulation of either of them was detected in the mother cell compartment, where their synthesis occurs. The other two components, 12.5 and 30 kDa, were generated 2 h later and were probably the products of posttranslational modifications of the two early forms occurring directly on the coat surface during spore maturation. None of the CotC forms was found either on the spore coat or in the mother cell compartment of a cotH mutant. This indicates that CotH serves a dual role of stabilizing the early forms of CotC and promoting the assembly of both early and late forms on the spore surface.

* Corresponding author. Mailing address: Dipartimento di Fisiologia Generale ed Ambientale, Università Federico II, via Mezzocannone 16, 80134 Naples, Italy. Phone: 39-081-2534636. Fax: 39-081-5514437. E-mail: ericca{at}unina.it.

{dagger} Present address: Gulbenkian Institute of Science, Oeiras, Portugal.

Journal of Bacteriology, February 2004, p. 1129-1135, Vol. 186, No. 4
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.4.1129-1135.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Isticato, R., Pelosi, A., Zilhao, R., Baccigalupi, L., Henriques, A. O., De Felice, M., Ricca, E. (2008). CotC-CotU Heterodimerization during Assembly of the Bacillus subtilis Spore Coat. J. Bacteriol. 190: 1267-1275 [Abstract] [Full Text]  
  • Wang, L., Perpich, J., Driks, A., Kroos, L. (2007). One Perturbation of the Mother Cell Gene Regulatory Network Suppresses the Effects of Another during Sporulation of Bacillus subtilis. J. Bacteriol. 189: 8467-8473 [Abstract] [Full Text]  
  • Costa, T., Serrano, M., Steil, L., Volker, U., Moran, C. P. Jr., Henriques, A. O. (2007). The Timing of cotE Expression Affects Bacillus subtilis Spore Coat Morphology but Not Lysozyme Resistance. J. Bacteriol. 189: 2401-2410 [Abstract] [Full Text]  
  • Veening, J.-W., Kuipers, O. P., Brul, S., Hellingwerf, K. J., Kort, R. (2006). Effects of Phosphorelay Perturbations on Architecture, Sporulation, and Spore Resistance in Biofilms of Bacillus subtilis.. J. Bacteriol. 188: 3099-3109 [Abstract] [Full Text]  
  • McPherson, D. C., Kim, H., Hahn, M., Wang, R., Grabowski, P., Eichenberger, P., Driks, A. (2005). Characterization of the Bacillus subtilis Spore Morphogenetic Coat Protein CotO. J. Bacteriol. 187: 8278-8290 [Abstract] [Full Text]  
  • Zilhao, R., Isticato, R., Martins, L. O., Steil, L., Volker, U., Ricca, E., Moran, C. P. Jr, Henriques, A. O. (2005). Assembly and Function of a Spore Coat-Associated Transglutaminase of Bacillus subtilis. J. Bacteriol. 187: 7753-7764 [Abstract] [Full Text]  
  • Baccigalupi, L., Castaldo, G., Cangiano, G., Isticato, R., Marasco, R., De Felice, M., Ricca, E. (2004). GerE-independent expression of cotH leads to CotC accumulation in the mother cell compartment during Bacillus subtilis sporulation. Microbiology 150: 3441-3449 [Abstract] [Full Text]  
  • Ragkousi, K., Setlow, P. (2004). Transglutaminase-Mediated Cross-Linking of GerQ in the Coats of Bacillus subtilis Spores. J. Bacteriol. 186: 5567-5575 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»