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Journal of Bacteriology, February 2004, p. 928-937, Vol. 186, No. 4
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.4.928-937.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Inhibition of Membrane-Bound Methane Monooxygenase and Ammonia Monooxygenase by Diphenyliodonium: Implications for Electron Transfer

Andrew K. Shiemke,1* Daniel J. Arp,2 and Luis A. Sayavedra-Soto2

Department of Biochemistry and Molecular Pharmacology, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown, West Virginia 26506-9142,1 Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331-29022

Received 16 September 2003/ Accepted 7 November 2003

Diphenyliodonium (DPI) is known to irreversibly inactivate flavoproteins. We have found that DPI inhibits both membrane-bound methane monooxygenase (pMMO) from Methylococcus capsulatus and ammonia monooxygenase (AMO) of Nitrosomonas europaea. The effect of DPI on NADH-dependent pMMO activity in vitro is ascribed to inactivation of NDH-2, a flavoprotein which we proposed catalyzes reduction of the quinone pool by NADH. DPI is a potent inhibitor of type 2 NADH:quinone oxidoreductase (NDH-2), with 50% inhibition occurring at {approx}5 µM. Inhibition of NDH-2 is irreversible and requires NADH. Inhibition of NADH-dependent pMMO activity by DPI in vitro is concomitant with inhibition of NDH-2, consistent with our proposal that NDH-2 mediates reduction of pMMO. Unexpectedly, DPI also inhibits pMMO activity driven by exogenous hydroquinols, but with {approx}100 µM DPI required to achieve 50% inhibition. Similar concentrations of DPI are required to inhibit formate-, formaldehyde-, and hydroquinol-driven pMMO activities in whole cells. The pMMO activity in DPI-treated cells greatly exceeds the activity of NDH-2 or pMMO in membranes isolated from those cells, suggesting that electron transfer from formate to pMMO in vivo can occur independent of NADH and NDH-2. AMO activity, which is known to be independent of NADH, is affected by DPI in a manner analogous to pMMO in vivo: {approx}100 µM is required for 50% inhibition regardless of the nature of the reducing agent. DPI does not affect hydroxylamine oxidoreductase activity and does not require AMO turnover to exert its inhibitory effect. Implications of these data for the electron transfer pathway from the quinone pool to pMMO and AMO are discussed.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Pharmacology, Robert C. Byrd Health Sciences Center, P.O. Box 9142, West Virginia University School of Medicine, Morgantown, WV 26506-9142. Phone: (304) 293-2310. Fax: (304) 293-6846. E-mail: ashiemke{at}hsc.wvu.edu.

Journal of Bacteriology, February 2004, p. 928-937, Vol. 186, No. 4
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.4.928-937.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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