This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schembri, M. A.
Right arrow Articles by Klemm, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schembri, M. A.
Right arrow Articles by Klemm, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2004, p. 1249-1257, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1249-1257.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Capsule Shields the Function of Short Bacterial Adhesins

Mark A. Schembri,1 Dorte Dalsgaard,1 and Per Klemm1*

Microbial Adhesion Group, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark1

Received 26 September 2003/ Accepted 26 November 2003

Bacterial surface structures such as capsules and adhesins are generally regarded as important virulence factors. Here we demonstrate that capsules block the function of the self-recognizing protein antigen 43 through physical shielding. The phenomenon is not restricted to Escherichia coli but can occur in other gram-negative bacteria. Likewise, we show that other short adhesins exemplified by the AIDA-I protein are blocked by the presence of a capsule. The results support the notion that capsule polysaccharides sterically prevent receptor-target recognition of short bacterial adhesins. This negative interference has important biological consequences, such as affecting the ability of bacteria to form biofilms.

* Corresponding author. Mailing address: Section of Molecular Microbiology, BioCentrum-DTU, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. Phone: 45 45 25 25 06. Fax: 45 45 93 28 09. E-mail: pkl{at}biocentrum.dtu.dk.

Journal of Bacteriology, March 2004, p. 1249-1257, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1249-1257.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Berry, R. E., Klumpp, D. J., Schaeffer, A. J. (2009). Urothelial Cultures Support Intracellular Bacterial Community Formation by Uropathogenic Escherichia coli. Infect. Immun. 77: 2762-2772 [Abstract] [Full Text]  
  • Corcionivoschi, N., Clyne, M., Lyons, A., Elmi, A., Gundogdu, O., Wren, B. W., Dorrell, N., Karlyshev, A. V., Bourke, B. (2009). Campylobacter jejuni Cocultured with Epithelial Cells Reduces Surface Capsular Polysaccharide Expression. Infect. Immun. 77: 1959-1967 [Abstract] [Full Text]  
  • Vejborg, R. M., Klemm, P. (2009). Cellular chain formation in Escherichia coli biofilms. Microbiology 155: 1407-1417 [Abstract] [Full Text]  
  • Leduc, I., Olsen, B., Elkins, C. (2009). Localization of the Domains of the Haemophilus ducreyi Trimeric Autotransporter DsrA Involved in Serum Resistance and Binding to the Extracellular Matrix Proteins Fibronectin and Vitronectin. Infect. Immun. 77: 657-666 [Abstract] [Full Text]  
  • Wu, M.-F., Yang, C.-Y., Lin, T.-L., Wang, J.-T., Yang, F.-L., Wu, S.-H., Hu, B.-S., Chou, T.-Y., Tsai, M.-D., Lin, C.-H., Hsieh, S.-L. (2009). Humoral Immunity against Capsule Polysaccharide Protects the Host from magA+ Klebsiella pneumoniae-Induced Lethal Disease by Evading Toll-Like Receptor 4 Signaling. Infect. Immun. 77: 615-621 [Abstract] [Full Text]  
  • Hart, E., Yang, J., Tauschek, M., Kelly, M., Wakefield, M. J., Frankel, G., Hartland, E. L., Robins-Browne, R. M. (2008). RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium. Infect. Immun. 76: 5247-5256 [Abstract] [Full Text]  
  • Munoz-Elias, E. J., Marcano, J., Camilli, A. (2008). Isolation of Streptococcus pneumoniae Biofilm Mutants and Their Characterization during Nasopharyngeal Colonization. Infect. Immun. 76: 5049-5061 [Abstract] [Full Text]  
  • Stukalov, O., Korenevsky, A., Beveridge, T. J., Dutcher, J. R. (2008). Use of Atomic Force Microscopy and Transmission Electron Microscopy for Correlative Studies of Bacterial Capsules. Appl. Environ. Microbiol. 74: 5457-5465 [Abstract] [Full Text]  
  • Shifrin, Y., Peleg, A., Ilan, O., Nadler, C., Kobi, S., Baruch, K., Yerushalmi, G., Berdichevsky, T., Altuvia, S., Elgrably-Weiss, M., Abe, C., Knutton, S., Sasakawa, C., Ritchie, J. M., Waldor, M. K., Rosenshine, I. (2008). Transient Shielding of Intimin and the Type III Secretion System of Enterohemorrhagic and Enteropathogenic Escherichia coli by a Group 4 Capsule. J. Bacteriol. 190: 5063-5074 [Abstract] [Full Text]  
  • Gualdi, L., Tagliabue, L., Bertagnoli, S., Ierano, T., De Castro, C., Landini, P. (2008). Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli. Microbiology 154: 2017-2024 [Abstract] [Full Text]  
  • Valle, J., Mabbett, A. N., Ulett, G. C., Toledo-Arana, A., Wecker, K., Totsika, M., Schembri, M. A., Ghigo, J.-M., Beloin, C. (2008). UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli. J. Bacteriol. 190: 4147-4161 [Abstract] [Full Text]  
  • Denou, E., Pridmore, R. D., Berger, B., Panoff, J.-M., Arigoni, F., Brussow, H. (2008). Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis. J. Bacteriol. 190: 3161-3168 [Abstract] [Full Text]  
  • Ong, C.-L. Y., Ulett, G. C., Mabbett, A. N., Beatson, S. A., Webb, R. I., Monaghan, W., Nimmo, G. R., Looke, D. F., McEwan, A. G., Schembri, M. A. (2008). Identification of Type 3 Fimbriae in Uropathogenic Escherichia coli Reveals a Role in Biofilm Formation. J. Bacteriol. 190: 1054-1063 [Abstract] [Full Text]  
  • Ulett, G. C., Valle, J., Beloin, C., Sherlock, O., Ghigo, J.-M., Schembri, M. A. (2007). Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract. Infect. Immun. 75: 3233-3244 [Abstract] [Full Text]  
  • Lloyd, A. L., Rasko, D. A., Mobley, H. L. T. (2007). Defining Genomic Islands and Uropathogen-Specific Genes in Uropathogenic Escherichia coli. J. Bacteriol. 189: 3532-3546 [Abstract] [Full Text]  
  • Ferrieres, L., Aslam, S. N., Cooper, R. M., Clarke, D. J. (2007). The yjbEFGH locus in Escherichia coli K-12 is an operon encoding proteins involved in exopolysaccharide production. Microbiology 153: 1070-1080 [Abstract] [Full Text]  
  • Scorpio, A., Chabot, D. J., Day, W. A., O'Brien, D. K., Vietri, N. J., Itoh, Y., Mohamadzadeh, M., Friedlander, A. M. (2007). Poly-{gamma}-Glutamate Capsule-Degrading Enzyme Treatment Enhances Phagocytosis and Killing of Encapsulated Bacillus anthracis. Antimicrob. Agents Chemother. 51: 215-222 [Abstract] [Full Text]  
  • Valle, J., Da Re, S., Henry, N., Fontaine, T., Balestrino, D., Latour-Lambert, P., Ghigo, J.-M. (2006). Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide. Proc. Natl. Acad. Sci. USA 103: 12558-12563 [Abstract] [Full Text]  
  • Davey, M. E., Duncan, M. J. (2006). Enhanced Biofilm Formation and Loss of Capsule Synthesis: Deletion of a Putative Glycosyltransferase in Porphyromonas gingivalis.. J. Bacteriol. 188: 5510-5523 [Abstract] [Full Text]  
  • Ulett, G. C., Webb, R. I., Schembri, M. A. (2006). Antigen-43-mediated autoaggregation impairs motility in Escherichia coli. Microbiology 152: 2101-2110 [Abstract] [Full Text]  
  • Beloin, C., Michaelis, K., Lindner, K., Landini, P., Hacker, J., Ghigo, J.-M., Dobrindt, U. (2006). The Transcriptional Antiterminator RfaH Represses Biofilm Formation in Escherichia coli. J. Bacteriol. 188: 1316-1331 [Abstract] [Full Text]  
  • Waldemarsson, J., Areschoug, T., Lindahl, G., Johnsson, E. (2006). The Streptococcal Blr and Slr Proteins Define a Family of Surface Proteins with Leucine-Rich Repeats: Camouflaging by Other Surface Structures. J. Bacteriol. 188: 378-388 [Abstract] [Full Text]  
  • Jenney, A. W., Clements, A., Farn, J. L., Wijburg, O. L., McGlinchey, A., Spelman, D. W., Pitt, T. L., Kaufmann, M. E., Liolios, L., Moloney, M. B., Wesselingh, S. L., Strugnell, R. A. (2006). Seroepidemiology of Klebsiella pneumoniae in an Australian Tertiary Hospital and Its Implications for Vaccine Development. J. Clin. Microbiol. 44: 102-107 [Abstract] [Full Text]  
  • Martinez, L. R., Casadevall, A. (2005). Specific Antibody Can Prevent Fungal Biofilm Formation and This Effect Correlates with Protective Efficacy. Infect. Immun. 73: 6350-6362 [Abstract] [Full Text]  
  • Schembri, M. A., Blom, J., Krogfelt, K. A., Klemm, P. (2005). Capsule and Fimbria Interaction in Klebsiella pneumoniae. Infect. Immun. 73: 4626-4633 [Abstract] [Full Text]  
  • Sherlock, O., Vejborg, R. M., Klemm, P. (2005). The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and Biofilm Formation. Infect. Immun. 73: 1954-1963 [Abstract] [Full Text]  
  • Schwan, W. R., Beck, M. T., Hultgren, S. J., Pinkner, J., Woolever, N. L., Larson, T. (2005). Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to D-Mannose Receptors. Infect. Immun. 73: 1226-1231 [Abstract] [Full Text]  
  • Sherlock, O., Schembri, M. A., Reisner, A., Klemm, P. (2004). Novel Roles for the AIDA Adhesin from Diarrheagenic Escherichia coli: Cell Aggregation and Biofilm Formation. J. Bacteriol. 186: 8058-8065 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»