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Journal of Bacteriology, March 2004, p. 1320-1329, Vol. 186, No. 5
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.5.1320-1329.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Functionally Critical Elements of CooA-Related CO Sensors
Hwan Youn, Robert L. Kerby, Mary Conrad, and Gary P. Roberts*
Department of Bacteriology, University of WisconsinMadison, Madison, Wisconsin 53706
Received 2 October 2003/
Accepted 24 November 2003
CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Further in vitro analysis of two CooA homologs from Azotobacter vinelandii and Carboxydothermus hydrogenoformans corroborated the in vivo data by revealing the ability of CO to bind to these hemoproteins and stimulate their binding at specific DNA sequences. These data, as well as the patterns of conserved residues in the homologs, are compared to what is already known about functionally important residues in the CooA protein of R. rubrum. The results identify critical regions of CooA and indicate features that distinguish CooAs from the general family of cyclic AMP receptor proteins.
* Corresponding author. Mailing address: Department of Bacteriology, University of WisconsinMadison, 420 Henry Mall, Madison, WI 53706. Phone: (608) 262-3567. Fax: (608) 262-9865. E-mail: groberts{at}bact.wisc.edu.
Journal of Bacteriology, March 2004, p. 1320-1329, Vol. 186, No. 5
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.5.1320-1329.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
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Copyright © 2004 by the American Society for Microbiology. All rights reserved.