This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uenoyama, A.
Right arrow Articles by Miyata, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uenoyama, A.
Right arrow Articles by Miyata, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2004, p. 1537-1545, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1537-1545.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of a 349-Kilodalton Protein (Gli349) Responsible for Cytadherence and Glass Binding during Gliding of Mycoplasma mobile

Atsuko Uenoyama,1 Akiko Kusumoto,1,{dagger} and Makoto Miyata1,2*

Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585,1 PRESTO, JST, Osaka, Japan2

Received 30 July 2003/ Accepted 5 November 2003

Several mycoplasma species are known to glide in the direction of the membrane protrusion (head-like structure), but the mechanism underlying this movement is entirely unknown. To identify proteins involved in the gliding mechanism, protein fractions of Mycoplasma mobile were analyzed for 10 gliding mutants isolated previously. One large protein (Gli349) was observed to be missing in a mutant m13 deficient in hemadsorption and glass binding. The predicted amino acid sequence indicated a 348,758-Da protein that was truncated at amino acid residue 1257 in the mutant. Immunofluorescence microscopy with a monoclonal antibody showed that Gli349 is localized at the head-like protrusion's base, which we designated the cell neck, and immunoelectron microscopy established that the Gli349 molecules are distributed all around this neck. The number of Gli349 molecules on a cell was estimated by immunoblot analysis to be 450 ± 200. The antibody inhibited both the hemadsorption and glass binding of M. mobile. When the antibody was used to treat gliding mycoplasmas, the gliding speed and the extent of glass binding were inhibited to similar extents depending on the concentration of the antibody. This suggested that the Gli349 molecule is involved not only in glass binding for gliding but also in movement. To explain the present results, a model for the mechanical cycle of gliding is discussed.

* Corresponding author. Mailing address: Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan. Phone: 81 (6) 6605 3157. Fax: 81 (6) 6605 3158. E-mail: miyata{at}sci.osaka-cu.ac.jp.

{dagger} Present address: Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan.

Journal of Bacteriology, March 2004, p. 1537-1545, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1537-1545.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakane, D., Miyata, M. (2009). Cytoskeletal Asymmetrical Dumbbell Structure of a Gliding Mycoplasma, Mycoplasma gallisepticum, Revealed by Negative-Staining Electron Microscopy. J. Bacteriol. 191: 3256-3264 [Abstract] [Full Text]  
  • Uenoyama, A., Seto, S., Nakane, D., Miyata, M. (2009). Regions on Gli349 and Gli521 Protein Molecules Directly Involved in Movements of Mycoplasma mobile Gliding Machinery, Suggested by Use of Inhibitory Antibodies and Mutants. J. Bacteriol. 191: 1982-1985 [Abstract] [Full Text]  
  • Hatchel, J. M., Balish, M. F. (2008). Attachment organelle ultrastructure correlates with phylogeny, not gliding motility properties, in Mycoplasma pneumoniae relatives. Microbiology 154: 286-295 [Abstract] [Full Text]  
  • Nakane, D., Miyata, M. (2007). Cytoskeletal "jellyfish" structure of Mycoplasma mobile. Proc. Natl. Acad. Sci. USA 104: 19518-19523 [Abstract] [Full Text]  
  • Burgos, R., Pich, O. Q., Querol, E., Pinol, J. (2007). Functional Analysis of the Mycoplasma genitalium MG312 Protein Reveals a Specific Requirement of the MG312 N-Terminal Domain for Gliding Motility. J. Bacteriol. 189: 7014-7023 [Abstract] [Full Text]  
  • Hiratsuka, Y., Miyata, M., Tada, T., Uyeda, T. Q. P. (2006). A microrotary motor powered by bacteria. Proc. Natl. Acad. Sci. USA 103: 13618-13623 [Abstract] [Full Text]  
  • Hasselbring, B. M., Page, C. A., Sheppard, E. S., Krause, D. C. (2006). Transposon Mutagenesis Identifies Genes Associated with Mycoplasma pneumoniae Gliding Motility.. J. Bacteriol. 188: 6335-6345 [Abstract] [Full Text]  
  • Nagai, R., Miyata, M. (2006). Gliding Motility of Mycoplasma mobile Can Occur by Repeated Binding to N-Acetylneuraminyllactose (Sialyllactose) Fixed on Solid Surfaces.. J. Bacteriol. 188: 6469-6475 [Abstract] [Full Text]  
  • Adan-Kubo, J., Uenoyama, A., Arata, T., Miyata, M. (2006). Morphology of Isolated Gli349, a Leg Protein Responsible for Mycoplasma mobile Gliding via Glass Binding, Revealed by Rotary Shadowing Electron Microscopy.. J. Bacteriol. 188: 2821-2828 [Abstract] [Full Text]  
  • Braun, T. F., Khubbar, M. K., Saffarini, D. A., McBride, M. J. (2005). Flavobacterium johnsoniae Gliding Motility Genes Identified by mariner Mutagenesis. J. Bacteriol. 187: 6943-6952 [Abstract] [Full Text]  
  • Charon, N. W. (2005). Mycoplasma takes a walk. Proc. Natl. Acad. Sci. USA 102: 13713-13714 [Full Text]  
  • Hasselbring, B. M., Jordan, J. L., Krause, D. C. (2005). Mutant Analysis Reveals a Specific Requirement for Protein P30 in Mycoplasma pneumoniae Gliding Motility. J. Bacteriol. 187: 6281-6289 [Abstract] [Full Text]  
  • Uenoyama, A., Miyata, M. (2005). From the Cover: Gliding ghosts of Mycoplasma mobile. Proc. Natl. Acad. Sci. USA 102: 12754-12758 [Abstract] [Full Text]  
  • Uenoyama, A., Miyata, M. (2005). Identification of a 123-Kilodalton Protein (Gli123) Involved in Machinery for Gliding Motility of Mycoplasma mobile. J. Bacteriol. 187: 5578-5584 [Abstract] [Full Text]  
  • Seto, S., Uenoyama, A., Miyata, M. (2005). Identification of a 521-Kilodalton Protein (Gli521) Involved in Force Generation or Force Transmission for Mycoplasma mobile Gliding. J. Bacteriol. 187: 3502-3510 [Abstract] [Full Text]  
  • Seto, S., Kenri, T., Tomiyama, T., Miyata, M. (2005). Involvement of P1 Adhesin in Gliding Motility of Mycoplasma pneumoniae as Revealed by the Inhibitory Effects of Antibody under Optimized Gliding Conditions. J. Bacteriol. 187: 1875-1877 [Abstract] [Full Text]  
  • Kusumoto, A., Seto, S., Jaffe, J. D., Miyata, M. (2004). Cell surface differentiation of Mycoplasma mobile visualized by surface protein localization. Microbiology 150: 4001-4008 [Abstract] [Full Text]  
  • Jaffe, J. D., Stange-Thomann, N., Smith, C., DeCaprio, D., Fisher, S., Butler, J., Calvo, S., Elkins, T., FitzGerald, M. G., Hafez, N., Kodira, C. D., Major, J., Wang, S., Wilkinson, J., Nicol, R., Nusbaum, C., Birren, B., Berg, H. C., Church, G. M. (2004). The Complete Genome and Proteome of Mycoplasma mobile. Genome Res 14: 1447-1461 [Abstract] [Full Text]  
  • Jaffe, J. D., Miyata, M., Berg, H. C. (2004). Energetics of Gliding Motility in Mycoplasma mobile. J. Bacteriol. 186: 4254-4261 [Abstract] [Full Text]  
  • Miyata, M., Petersen, J. D. (2004). Spike Structure at the Interface between Gliding Mycoplasma mobile Cells and Glass Surfaces Visualized by Rapid-Freeze-and-Fracture Electron Microscopy. J. Bacteriol. 186: 4382-4386 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»