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 Previous Article

Journal of Bacteriology, March 2004, p. 1574-1578, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1574-1578.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

VpsT Is a Transcriptional Regulator Required for Expression of vps Biosynthesis Genes and the Development of Rugose Colonial Morphology in Vibrio cholerae O1 El Tor

Catharina Casper-Lindley and Fitnat H. Yildiz*

Department of Environmental Toxicology, University of California at Santa Cruz, Santa Cruz, California 95064

Received 17 June 2003/ Accepted 25 November 2003

Vibrio cholerae switches between smooth and rugose colonial variants. The rugose variant produces more vibrio polysaccharides (VPSEl Tor) and forms well-developed biofilms. Both phenotypes depend on expression of vps biosynthesis genes. We identified a positive transcriptional regulator of vps gene expression, VpsT, which is homologous to response regulators of two-component regulatory systems. Disruption of vpsT in the rugose variant yields smooth colonies, prevents formation of mature biofilms, and decreases vps gene expression. The interaction between VpsT and VpsR, a previously identified positive regulator of vps genes, was also investigated.

* Corresponding author. Mailing address: Department of Environmental Toxicology, Jack Baskin Engineering, Room 269, University of California, Santa Cruz, Santa Cruz, CA 95064. Phone: (831) 459-1588. Fax: (831) 459-3524. E-mail: yildiz{at}etox.ucsc.edu.

Journal of Bacteriology, March 2004, p. 1574-1578, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1574-1578.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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