This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brinsmade, S. R. Articles by Escalante-Semerena, J. C. Search for Related Content PubMed PubMed Citation Articles by Brinsmade, S. R. Articles by Escalante-Semerena, J. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2004, p. 1890-1892, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1890-1892.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The eutD Gene of Salmonella enterica Encodes a Protein with Phosphotransacetylase Enzyme Activity

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin

Received 17 November 2003/ Accepted 9 December 2003

The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.

This article has been cited by other articles:

• Bologna, F. P., Andreo, C. S., Drincovich, M. F. (2007). Escherichia coli Malic Enzymes: Two Isoforms with Substantial Differences in Kinetic Properties, Metabolic Regulation, and Structure. J. Bacteriol. 189: 5937-5946 [Abstract] [Full Text]  
• Brinsmade, S. R., Escalante-Semerena, J. C. (2007). In Vivo and in Vitro Analyses of Single-amino Acid Variants of the Salmonella enterica Phosphotransacetylase Enzyme Provide Insights into the Function of Its N-terminal Domain. J. Biol. Chem. 282: 12629-12640 [Abstract] [Full Text]  
• Xu, P., Alves, J. M., Kitten, T., Brown, A., Chen, Z., Ozaki, L. S., Manque, P., Ge, X., Serrano, M. G., Puiu, D., Hendricks, S., Wang, Y., Chaplin, M. D., Akan, D., Paik, S., Peterson, D. L., Macrina, F. L., Buck, G. A. (2007). Genome of the Opportunistic Pathogen Streptococcus sanguinis. J. Bacteriol. 189: 3166-3175 [Abstract] [Full Text]  
• Liu, Y., Leal, N. A., Sampson, E. M., Johnson, C. L. V., Havemann, G. D., Bobik, T. A. (2007). PduL Is an Evolutionarily Distinct Phosphotransacylase Involved in B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica Serovar Typhimurium LT2. J. Bacteriol. 189: 1589-1596 [Abstract] [Full Text]  
• Buan, N. R., Rehfeld, K., Escalante-Semerena, J. C. (2006). Studies of the CobA-Type ATP:Co(I)rrinoid Adenosyltransferase Enzyme of Methanosarcina mazei Strain Go1.. J. Bacteriol. 188: 3543-3550 [Abstract] [Full Text]  
• Penrod, J. T., Roth, J. R. (2006). Conserving a Volatile Metabolite: a Role for Carboxysome-Like Organelles in Salmonella enterica.. J. Bacteriol. 188: 2865-2874 [Abstract] [Full Text]  
• Buan, N. R., Escalante-Semerena, J. C. (2005). Computer-assisted Docking of Flavodoxin with the ATP:Co(I)rrinoid Adenosyltransferase (CobA) Enzyme Reveals Residues Critical for Protein-Protein Interactions but Not for Catalysis. J. Biol. Chem. 280: 40948-40956 [Abstract] [Full Text]  
• Brinsmade, S. R., Paldon, T., Escalante-Semerena, J. C. (2005). Minimal Functions and Physiological Conditions Required for Growth of Salmonella enterica on Ethanolamine in the Absence of the Metabolosome. J. Bacteriol. 187: 8039-8046 [Abstract] [Full Text]  
• Starai, V. J., Garrity, J., Escalante-Semerena, J. C. (2005). Acetate excretion during growth of Salmonella enterica on ethanolamine requires phosphotransacetylase (EutD) activity, and acetate recapture requires acetyl-CoA synthetase (Acs) and phosphotransacetylase (Pta) activities. Microbiology 151: 3793-3801 [Abstract] [Full Text]  
• Price-Carter, M., Fazzio, T. G., Vallbona, E. I., Roth, J. R. (2005). Polyphosphate Kinase Protects Salmonella enterica from Weak Organic Acid Stress. J. Bacteriol. 187: 3088-3099 [Abstract] [Full Text]  
• Sheppard, D. E., Penrod, J. T., Bobik, T., Kofoid, E., Roth, J. R. (2004). Evidence that a B12-Adenosyl Transferase Is Encoded within the Ethanolamine Operon of Salmonella enterica. J. Bacteriol. 186: 7635-7644 [Abstract] [Full Text]  
• Penrod, J. T., Mace, C. C., Roth, J. R. (2004). A pH-Sensitive Function and Phenotype: Evidence that EutH Facilitates Diffusion of Uncharged Ethanolamine in Salmonella enterica. J. Bacteriol. 186: 6885-6890 [Abstract] [Full Text]