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Journal of Bacteriology, April 2004, p. 1972-1982, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.1972-1982.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sortase B, a New Class of Sortase in Listeria monocytogenes

Hélène Bierne,1,{dagger} Caroline Garandeau,2,{dagger} M. Graciela Pucciarelli,3 Christophe Sabet,1 Salete Newton,4 Francisco Garcia-del Portillo,3 Pascale Cossart,1* and Alain Charbit2*

Unité des Interactions Bactéries-Cellules, Institut Pasteur, 75724 Paris Cedex 15,1 INSERM U-570, CHU Necker-Enfants Malades, 75730 Paris Cedex 15, France,2 Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia-CSIC, Campus de Cantoblanco, 28049, Madrid. Spain,3 Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 730194

Received 7 July 2003/ Accepted 2 December 2003

Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. Here, we focus on the role of the second sortase, SrtB. Whereas SrtA acts on most of the proteins in the peptidoglycan fraction, SrtB appears to target minor amounts of surface polypeptides. We identified one of the SrtB-anchored proteins as the virulence factor SvpA, a surface-exposed protein which does not contain the LPXTG motif. Therefore, as in Staphylococcus aureus, the listerial SrtB represents a second class of sortase in L. monocytogenes, involved in the attachment of a subset of proteins to the cell wall, most likely by recognizing an NXZTN sorting motif. The {Delta}srtB mutant strain does not have defects in bacterial entry, growth, or motility in tissue-cultured cells and does not show attenuated virulence in mice. SrtB-mediated anchoring could therefore be required to anchor surface proteins involved in the adaptation of this microorganism to different environmental conditions.

* Corresponding author. Mailing address for Pascale Cossart: Unité des Interactions Bactéries-Cellules, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 88 41. Fax: 33 1 45 68 87 06. E-mail: pcossart{at}pasteur.fr. Mailing address for Alain Charbit: INSERM U-570, CHU Necker-Enfants Malades, 156, rue de Vaugirard, 75730 Paris Cedex 15, France. Phone: 33 1 40 61 53 76. Fax: 33 1 40 61 55 92. E-mail: charbit{at}necker.fr.

{dagger} H.B. and C.G. contributed equally to this work.

Journal of Bacteriology, April 2004, p. 1972-1982, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.1972-1982.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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