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Journal of Bacteriology, April 2004, p. 2091-2098, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.2091-2098.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Protective Role for H-NS Protein in IS1 Transposition

Claudine Rouquette,{dagger} Marie-Claude Serre,{ddagger} and David Lane*

Laboratoire de Microbiologie et Génétique Moléculaire, CNRS, Toulouse, France

Received 26 September 2003/ Accepted 12 December 2003

The transposase (InsAB') of the insertion element IS1 can create breaks in DNA that lead to induction of the SOS response. We have used the SOS response to InsAB' to screen for host mutations that affect InsAB' function and thus point to host functions that contribute to the IS1 transposition mechanism. Mutations in the hns gene, which codes for a DNA binding protein with wide-ranging effects on gene expression, abolish the InsAB'-induced SOS response. They also reduce transposition, whether by simple insertion or cointegrate formation, at least 100-fold compared with the frequency seen in hns+ cells. Examination of protein profiles revealed that in an hns-null mutant, InsAB' is undetectable under conditions where it constitutes the most abundant protein in hns+ cells. Likewise, brief labeling of the hns cells with [35S]methionine revealed very small amounts of InsAB', and this was undetectable after a short chase. Transcription from the promoters used to express insAB' was essentially unaltered in hns cells, as was the level of insAB' mRNA. A mutation in lon, but not in ftsH or clpP, restored InsAB' synthesis in the hns strain, and a mutation in ssrA partially restored it, implying that the absence of H-NS leads to a problem in completing translation of insAB' mRNA and/or degradation of nascent InsAB' protein.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Génétique Moléculaire, bÂt. IBCG, CNRS, 118 Route de Narbonne, Toulouse, France. Phone: (33) (0)5 61 33 59 68. Fax: (33) (0)5 61 33 58 86. E-mail: dave{at}ibcg.biotoul.fr.

{dagger} Present address: Chemin des Berges, 31290 Villenouvelle, France.

{ddagger} Present address: Institut de Génétique et Microbiologie, Université Paris-Sud/CNRS, Centre Universitaire d'Orsay, 91405 Orsay, France.

Journal of Bacteriology, April 2004, p. 2091-2098, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.2091-2098.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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