This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peters, F.
Right arrow Articles by Boll, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peters, F.
Right arrow Articles by Boll, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2004, p. 2156-2163, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.2156-2163.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Selenocysteine-Containing Proteins in Anaerobic Benzoate Metabolism of Desulfococcus multivorans

Franziska Peters,1 Michael Rother,2,{dagger} and Matthias Boll1*

Institut für Biologie II, Albert-Ludwigs-Universität, Freiburg,1 Mikrobiologie, Department für Biologie I, Ludwig-Maximilians-Universität, Munich, Germany2

Received 29 July 2003/ Accepted 11 December 2003

The sulfate-reducing bacterium Desulfococcus multivorans uses various aromatic compounds as sources of cell carbon and energy. In this work, we studied the initial steps in the aromatic metabolism of this strictly anaerobic model organism. An ATP-dependent benzoate coenzyme A (CoA) ligase (AMP plus PPi forming) composed of a single 59-kDa subunit was purified from extracts of cells grown on benzoate. Specific activity was highest with benzoate and some benzoate derivatives, whereas aliphatic carboxylic acids were virtually unconverted. The N-terminal amino acid sequence showed high similarities with benzoate CoA ligases from Thauera aromatica and Azoarcus evansii. When cultivated on benzoate, cells strictly required selenium and molybdenum, whereas growth on nonaromatic compounds, such as cyclohexanecarboxylate or lactate, did not depend on the presence of the two trace elements. The growth rate on benzoate was half maximal with 1 nM selenite present in the growth medium. In molybdenum- and/or selenium-depleted cultures, growth on benzoate could be induced by addition of the missing trace elements. In extracts of cells grown on benzoate in the presence of [75Se]selenite, three radioactively labeled proteins with molecular masses of ~100, 30, and 27 kDa were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The 100- and 30-kDa selenoproteins were 5- to 10-fold induced in cells grown on benzoate compared to cells grown on lactate. These results suggest that the dearomatization process in D. multivorans is not catalyzed by the ATP-dependent Fe-S enzyme benzoyl-CoA reductase as in facultative anaerobes but rather involves unknown molybdenum- and selenocysteine-containing proteins.

* Corresponding author. Mailing address: Mikrobiologie, Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany. Phone: 49 7612032685. Fax: 49 7612032626. E-mail: boll{at}biologie.uni-freiburg.de.

{dagger} Present address: Department of Microbiology, University of Illinois, Urbana-Champaign, Ill.

Journal of Bacteriology, April 2004, p. 2156-2163, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.2156-2163.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wischgoll, S., Taubert, M., Peters, F., Jehmlich, N., von Bergen, M., Boll, M. (2009). Decarboxylating and Nondecarboxylating Glutaryl-Coenzyme A Dehydrogenases in the Aromatic Metabolism of Obligately Anaerobic Bacteria. J. Bacteriol. 191: 4401-4409 [Abstract] [Full Text]  
  • Carmona, M., Zamarro, M. T., Blazquez, B., Durante-Rodriguez, G., Juarez, J. F., Valderrama, J. A., Barragan, M. J. L., Garcia, J. L., Diaz, E. (2009). Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View. Microbiol. Mol. Biol. Rev. 73: 71-133 [Abstract] [Full Text]  
  • Mouttaki, H., Nanny, M. A., McInerney, M. J. (2009). Metabolism of Hydroxylated and Fluorinated Benzoates by Syntrophus aciditrophicus and Detection of a Fluorodiene Metabolite. Appl. Environ. Microbiol. 75: 998-1004 [Abstract] [Full Text]  
  • Thiele, B., Rieder, O., Jehmlich, N., von Bergen, M., Muller, M., Boll, M. (2008). Aromatizing Cyclohexa-1,5-diene-1-carbonyl-Coenzyme A Oxidase: CHARACTERIZATION AND ITS ROLE IN ANAEROBIC AROMATIC METABOLISM. J. Biol. Chem. 283: 20713-20721 [Abstract] [Full Text]  
  • Durante-Rodriguez, G., Zamarro, M. T., Garcia, J. L., Diaz, E., Carmona, M. (2008). New insights into the BzdR-mediated transcriptional regulation of the anaerobic catabolism of benzoate in Azoarcus sp. CIB. Microbiology 154: 306-316 [Abstract] [Full Text]  
  • Peters, F., Shinoda, Y., McInerney, M. J., Boll, M. (2007). Cyclohexa-1,5-Diene-1-Carbonyl-Coenzyme A (CoA) Hydratases of Geobacter metallireducens and Syntrophus aciditrophicus: Evidence for a Common Benzoyl-CoA Degradation Pathway in Facultative and Strict Anaerobes. J. Bacteriol. 189: 1055-1060 [Abstract] [Full Text]  
  • Song, B., Ward, B. B. (2005). Genetic Diversity of Benzoyl Coenzyme A Reductase Genes Detected in Denitrifying Isolates and Estuarine Sediment Communities. Appl. Environ. Microbiol. 71: 2036-2045 [Abstract] [Full Text]  
  • Barragan, M. J. L., Diaz, E., Garcia, J. L., Carmona, M. (2004). Genetic clues on the evolution of anaerobic catabolism of aromatic compounds. Microbiology 150: 2018-2021 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»