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Multiple Formaldehyde Oxidation/Detoxification Pathways in Burkholderia fungorum LB400

Christopher J. Marx,^1, Jonathan A. Miller,¹ Ludmila Chistoserdova,² and Mary E. Lidstrom^1,2*

Department of Microbiology,¹ Department of Chemical Engineering, University of Washington, Seattle, Washington 98195-2180²

Received 1 September 2003/ Accepted 30 December 2003

Burkholderia species are free-living bacteria with a versatile metabolic lifestyle. The genome of B. fungorum LB400 is predicted to encode three different pathways for formaldehyde oxidation: an NAD-linked, glutathione (GSH)-independent formaldehyde dehydrogenase; an NAD-linked, GSH-dependent formaldehyde oxidation system; and a tetrahydromethanopterin-methanofuran-dependent formaldehyde oxidation system. The other Burkholderia species for which genome sequences are available, B. mallei, B. pseudomallei, and B. cepacia, are predicted to contain only the first two of these pathways. The roles of the three putative formaldehyde oxidation pathways in B. fungorum LB400 have been assessed via knockout mutations in each of these pathways, as well as in all combinations of knockouts. The resulting mutants have the expected loss of enzyme activities and exhibit defects of varying degrees of severity during growth on choline, a formaldehyde-producing substrate. Our data suggest that all three pathways are involved in formaldehyde detoxification and are functionally redundant under the tested conditions.

Present address: Michigan State University, East Lansing, MI 48824-4320.

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