This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kato, J.-y. Articles by Horinouchi, S. Search for Related Content PubMed PubMed Citation Articles by Kato, J.-y. Articles by Horinouchi, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2004, p. 2206-2211, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.2206-2211.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Single Target Is Sufficient To Account for the Biological Effects of the A-Factor Receptor Protein of Streptomyces griseus

Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan

Received 7 November 2003/ Accepted 23 December 2003

In the model of the A-factor (2-isocapryloyl-3R-hydroxymethyl--butyrolactone) regulatory cascade in Streptomyces griseus, A-factor binds ArpA, the A-factor receptor protein, that has bound to the adpA promoter and dissociates it from the DNA, thus inducing the transcription of adpA. AdpA switches on the transcription of a number of genes required for secondary metabolism and morphological differentiation, forming an AdpA regulon. Consistent with this model, arpA null mutants produced streptomycin and a yellow pigment in larger amounts and formed aerial hyphae from an earlier growth stage than the wild-type strain. On the other hand, mutant MK2, expressing a mutant ArpA (Trp119Ala), neither produced secondary metabolites nor formed aerial hyphae, because this A-factor-insensitive mutant ArpA always bound to and repressed the adpA promoter due to the amino acid replacement of Trp-119 with Ala. Introduction of adpA under the control of a foreign promoter into mutant MK2 restored all of the phenotypes that we could observe, which suggests that the only significant target of ArpA is adpA. In contrast to other -butyrolactone regulatory systems, disruption of arpA had no effect on A-factor production, indicating that ArpA does not regulate A-factor biosynthesis. Instead, A-factor production was found to be repressed by AdpA in a two-step regulatory feedback loop.

This article has been cited by other articles:

• Arakawa, K., Mochizuki, S., Yamada, K., Noma, T., Kinashi, H. (2007). {gamma}-Butyrolactone autoregulator-receptor system involved in lankacidin and lankamycin production and morphological differentiation in Streptomyces rochei. Microbiology 153: 1817-1827 [Abstract] [Full Text]  
• Tomono, A., Tsai, Y., Yamazaki, H., Ohnishi, Y., Horinouchi, S. (2005). Transcriptional Control by A-Factor of strR, the Pathway-Specific Transcriptional Activator for Streptomycin Biosynthesis in Streptomyces griseus. J. Bacteriol. 187: 5595-5604 [Abstract] [Full Text]  
• Ramos, J. L., Martinez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., Tobes, R. (2005). The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69: 326-356 [Abstract] [Full Text]  
• Kato, J.-y., Chi, W.-J., Ohnishi, Y., Hong, S.-K., Horinouchi, S. (2005). Transcriptional Control by A-Factor of Two Trypsin Genes in Streptomyces griseus. J. Bacteriol. 187: 286-295 [Abstract] [Full Text]