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Journal of Bacteriology, April 2004, p. 2295-2302, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2295-2302.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

GldI Is a Lipoprotein That Is Required for Flavobacterium johnsoniae Gliding Motility and Chitin Utilization

Mark J. McBride* and Timothy F. Braun

Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin 53201

Received 11 November 2003/ Accepted 8 January 2004

Cells of Flavobacterium johnsoniae glide rapidly over surfaces by an unknown mechanism. Seven genes (gldA, gldB, gldD, gldF, gldG, gldH, and ftsX) that are required for gliding motility have been described. Complementation of the nonmotile mutants UW102-41, UW102-85, and UW102-92 identified another gene, gldI, that is required for gliding motility. gldI mutants formed nonspreading colonies, and individual cells were completely nonmotile. They were also resistant to bacteriophages that infect wild-type cells, and they failed to digest chitin. Introduction of wild-type gldI on a plasmid restored colony spreading, cell motility, phage sensitivity, and the ability to digest chitin to the gldI mutants. gldI encodes a predicted 199-amino-acid protein that localized to the membrane fraction. Labeling studies with [3H]palmitate indicated that GldI is a lipoprotein. GldI is similar to peptidyl-prolyl cis/trans-isomerases of the FK506-binding protein family and may be involved in folding cell envelope protein components of the motility machinery.

* Corresponding author. Mailing address: Department of Biological Sciences, 181 Lapham Hall, University of Wisconsin—Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211. Phone: (414) 229-5844. Fax: (414) 229-3926. E-mail: mcbride{at}uwm.edu.

Journal of Bacteriology, April 2004, p. 2295-2302, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2295-2302.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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