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Journal of Bacteriology, April 2004, p. 2402-2412, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2402-2412.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Analysis of the Salmonella enterica Type III Secretion-Associated ATPase InvC Defines Discrete Functional Domains

Yukihiro Akeda and Jorge E. Galán*

Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536

Received 7 October 2003/ Accepted 12 January 2004

An essential component of all type III secretion systems is a highly conserved ATPase that shares significant amino acid sequence similarity to the ß subunit of the F0F1 ATPases and is thought to provide the energy for the secretion process. We have performed a genetic and functional analysis of InvC, the ATPase associated with the Salmonella enterica type III secretion system encoded within its pathogenicity island 1. Through a mutagenesis analysis, we have identified amino acid residues that are essential for specific activities of InvC, such as nucleotide hydrolysis and membrane binding. This has allowed us to define discrete domains of InvC that are specifically associated with different essential activities of this protein.

* Corresponding author. Mailing address: Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536. Phone: (203) 737-2404. Fax: (203) 737-2630. E-mail: jorge.galan{at}yale.edu.

Journal of Bacteriology, April 2004, p. 2402-2412, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2402-2412.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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