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Journal of Bacteriology, April 2004, p. 2476-2480, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2476-2480.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

PhoP Can Activate Its Target Genes in a PhoQ-Independent Manner

Sergio Lejona,1 María Eugenia Castelli,1 María Laura Cabeza,1 Linda J. Kenney,2 Eleonora García Véscovi,1* and Fernando C. Soncini1

Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, 2000 Rosario, Argentina,1 Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois 606122

Received 7 October 2003/ Accepted 7 January 2004

The PhoP/PhoQ two-component system controls the extracellular magnesium depletion response in Salmonella enterica. Previous studies have shown that PhoP is unable to up-regulate its target genes in the absence of PhoQ function. In this work, we demonstrate that PhoP overexpression can substitute for PhoQ- and phosphorylation-dependent activation. Either a high concentration of PhoP or activation via phosphorylation stimulates PhoP self-association.

* Corresponding author. Mailing address: Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR), Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Suipacha 531, S2002LRK-Rosario, Argentina. Phone: 54-341-4356369. Fax: 54-341-4390465. E-mail: pat-bact{at}citynet.net.ar.

Journal of Bacteriology, April 2004, p. 2476-2480, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2476-2480.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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