This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maughan, H. Articles by Nicholson, W. L. Search for Related Content PubMed PubMed Citation Articles by Maughan, H. Articles by Nicholson, W. L.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2004, p. 2481-2486, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2481-2486.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel rpoB Mutations Conferring Rifampin Resistance on Bacillus subtilis: Global Effects on Growth, Competence, Sporulation, and Germination

Departments of Ecology and Evolutionary Biology,¹ Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721²

Received 11 September 2003/ Accepted 30 December 2003

Previously, spontaneous rifampin resistance mutations were isolated in cluster I of the rpoB gene, resulting in amino acid replacements (Q469R, H482R, H482Y, or S487L) in the Bacillus subtilis RNA polymerase ß subunit (W. L. Nicholson and H. Maughan, J. Bacteriol. 184:4936-4940, 2002). In this study, each amino acid change in the ß subunit was observed to result in its own unique spectrum of effects on growth and various developmental events, including sporulation, germination, and competence for transformation. The results thus establish the important role played by the RNA polymerase ß subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis.

This article has been cited by other articles:

• Perkins, A. E., Nicholson, W. L. (2008). Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants. J. Bacteriol. 190: 807-814 [Abstract] [Full Text]  
• Mwangi, M. M., Wu, S. W., Zhou, Y., Sieradzki, K., de Lencastre, H., Richardson, P., Bruce, D., Rubin, E., Myers, E., Siggia, E. D., Tomasz, A. (2007). Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing. Proc. Natl. Acad. Sci. USA 104: 9451-9456 [Abstract] [Full Text]