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Journal of Bacteriology, April 2004, p. 2487-2491, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2487-2491.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Protein-Protein Interactions of Proteins from the ESAT-6 Family of Mycobacterium tuberculosis

Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, DK-2300, Denmark

Received 29 August 2003/ Accepted 13 January 2004

In the present study, we demonstrate that, in analogy with the genes encoding ESAT-6 and CFP-10, the genes rv0287 and rv0288 from the ESAT-6 gene family are cotranscribed. Using Western-Western blotting and protein-print overlay methodologies, we demonstrate that ESAT-6 and CFP-10, as well as the protein pair Rv0288/Rv0287, interact pairwise in a highly specific way. Most notably, the ESAT-6 proteins interact directly with Rv3873, a possible cell envelope component of the ESAT-6 secretion pathway.

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