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Analysis of an Engineered Salmonella Flagellar Fusion Protein, FliR-FlhB

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520

Received 17 November 2003/ Accepted 9 January 2004

Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.

This paper is dedicated to Robert Macnab, who died suddenly on 7 September 2003.

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