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Journal of Bacteriology, April 2004, p. 2495-2498, Vol. 186, No. 8
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.8.2495-2498.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Analysis of an Engineered Salmonella Flagellar Fusion Protein, FliR-FlhB
John S. Van Arnam, Jonathan L. McMurry, May Kihara,* and Robert M. Macnab
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520
Received 17 November 2003/
Accepted 9 January 2004
Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.
* Corresponding author. Mailing address: Yale University, Department of Molecular Biophysics and Biochemistry 0734, 266 Whitney Ave., P.O. Box 208114, New Haven, CT 06520-8114. Phone: (203) 432-5588. Fax: (203) 432-9782. E-mail:
may.macnab{at}yale.edu.
This paper is dedicated to Robert Macnab, who died suddenly on 7 September 2003.
Journal of Bacteriology, April 2004, p. 2495-2498, Vol. 186, No. 8
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.8.2495-2498.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
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