This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schirm, M. Articles by Logan, S. M. Search for Related Content PubMed PubMed Citation Articles by Schirm, M. Articles by Logan, S. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2004, p. 2523-2531, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2523-2531.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa

Department Chemistry, University of Montreal,¹ Caprion Pharmaceuticals, Montreal, Quebec,⁴ Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada,³ Department of Medicine/Infectious Disease, University of Florida, Gainesville, Florida²

Received 14 November 2003/ Accepted 21 January 2004

Type a flagellins from two strains of Pseudomonas aeruginosa, strains PAK and JJ692, were found to be glycosylated with unique glycan structures. In both cases, two sites of O-linked glycosylation were identified on each monomer, and these sites were localized to the central, surface-exposed domain of the monomer in the assembled filament. The PAK flagellin was modified with a heterogeneous glycan comprising up to 11 monosaccharide units that were O linked through a rhamnose residue to the protein backbone. The flagellin of JJ692 was less complex and had a single rhamnose substitution at each site. The role of the glycosylation island gene cluster in the production of each of these glycosyl moieties was investigated. These studies revealed that the orfA and orfN genes were required for attachment of the heterologous glycan and the proximal rhamnose residue, respectively.

This article has been cited by other articles:

• Miller, W. L., Matewish, M. J., McNally, D. J., Ishiyama, N., Anderson, E. M., Brewer, D., Brisson, J.-R., Berghuis, A. M., Lam, J. S. (2008). Flagellin Glycosylation in Pseudomonas aeruginosa PAK Requires the O-antigen Biosynthesis Enzyme WbpO. J. Biol. Chem. 283: 3507-3518 [Abstract] [Full Text]  
• Taguchi, F., Shibata, S., Suzuki, T., Ogawa, Y., Aizawa, S.-I., Takeuchi, K., Ichinose, Y. (2008). Effects of Glycosylation on Swimming Ability and Flagellar Polymorphic Transformation in Pseudomonas syringae pv. tabaci 6605. J. Bacteriol. 190: 764-768 [Abstract] [Full Text]  
• Takeuchi, K., Ono, H., Yoshida, M., Ishii, T., Katoh, E., Taguchi, F., Miki, R., Murata, K., Kaku, H., Ichinose, Y. (2007). Flagellin Glycans from Two Pathovars of Pseudomonas syringae Contain Rhamnose in D and L Configurations in Different Ratios and Modified 4-Amino-4,6-Dideoxyglucose. J. Bacteriol. 189: 6945-6956 [Abstract] [Full Text]  
• Sebaihia, M., Peck, M. W., Minton, N. P., Thomson, N. R., Holden, M. T.G., Mitchell, W. J., Carter, A. T., Bentley, S. D., Mason, D. R., Crossman, L., Paul, C. J., Ivens, A., Wells-Bennik, M. H.J., Davis, I. J., Cerdeno-Tarraga, A. M., Churcher, C., Quail, M. A., Chillingworth, T., Feltwell, T., Fraser, A., Goodhead, I., Hance, Z., Jagels, K., Larke, N., Maddison, M., Moule, S., Mungall, K., Norbertczak, H., Rabbinowitsch, E., Sanders, M., Simmonds, M., White, B., Whithead, S., Parkhill, J. (2007). Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes. Genome Res 17: 1082-1092 [Abstract] [Full Text]  
• Verma, A., Schirm, M., Arora, S. K., Thibault, P., Logan, S. M., Ramphal, R. (2006). Glycosylation of b-Type Flagellin of Pseudomonas aeruginosa: Structural and Genetic Basis.. J. Bacteriol. 188: 4395-4403 [Abstract] [Full Text]  
• Hitchen, P. G., Dell, A. (2006). Bacterial glycoproteomics. Microbiology 152: 1575-1580 [Abstract] [Full Text]  
• Logan, S. M. (2006). Flagellar glycosylation - a new component of the motility repertoire?. Microbiology 152: 1249-1262 [Abstract] [Full Text]  
• Prince, A. (2006). Flagellar Activation of Epithelial Signaling. Am. J. Respir. Cell Mol. Bio. 34: 548-551 [Abstract] [Full Text]  
• Schoenhofen, I. C., Lunin, V. V., Julien, J.-P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.-R., Aubry, A., Logan, S. M., Bhatia, S., Wakarchuk, W. W., Young, N. M. (2006). Structural and Functional Characterization of PseC, an Aminotransferase Involved in the Biosynthesis of Pseudaminic Acid, an Essential Flagellar Modification in Helicobacter pylori. J. Biol. Chem. 281: 8907-8916 [Abstract] [Full Text]  
• Tseng, J., Do, J., Widdicombe, J. H., Machen, T. E. (2006). Innate immune responses of human tracheal epithelium to Pseudomonas aeruginosa flagellin, TNF-{alpha}, and IL-1beta. Am. J. Physiol. Cell Physiol. 290: C678-C690 [Abstract] [Full Text]  
• Smedley, J. G. III, Jewell, E., Roguskie, J., Horzempa, J., Syboldt, A., Stolz, D. B., Castric, P. (2005). Influence of Pilin Glycosylation on Pseudomonas aeruginosa 1244 Pilus Function. Infect. Immun. 73: 7922-7931 [Abstract] [Full Text]  
• Verma, A., Arora, S. K., Kuravi, S. K., Ramphal, R. (2005). Roles of Specific Amino Acids in the N Terminus of Pseudomonas aeruginosa Flagellin and of Flagellin Glycosylation in the Innate Immune Response. Infect. Immun. 73: 8237-8246 [Abstract] [Full Text]  
• Eichler, J., Adams, M. W. W. (2005). Posttranslational Protein Modification in Archaea. Microbiol. Mol. Biol. Rev. 69: 393-425 [Abstract] [Full Text]  
• Arora, S. K., Neely, A. N., Blair, B., Lory, S., Ramphal, R. (2005). Role of Motility and Flagellin Glycosylation in the Pathogenesis of Pseudomonas aeruginosa Burn Wound Infections. Infect. Immun. 73: 4395-4398 [Abstract] [Full Text]  
• Voisin, S., Houliston, R. S., Kelly, J., Brisson, J.-R., Watson, D., Bardy, S. L., Jarrell, K. F., Logan, S. M. (2005). Identification and Characterization of the Unique N-Linked Glycan Common to the Flagellins and S-layer Glycoprotein of Methanococcus voltae. J. Biol. Chem. 280: 16586-16593 [Abstract] [Full Text]  
• Schirm, M., Kalmokoff, M., Aubry, A., Thibault, P., Sandoz, M., Logan, S. M. (2004). Flagellin from Listeria monocytogenes Is Glycosylated with {beta}-O-Linked N-Acetylglucosamine. J. Bacteriol. 186: 6721-6727 [Abstract] [Full Text]  
• Messner, P. (2004). Prokaryotic Glycoproteins: Unexplored but Important. J. Bacteriol. 186: 2517-2519 [Full Text]