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Journal of Bacteriology, May 2004, p. 2540-2547, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2540-2547.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of the Alternative Sigma Factor {sigma}54 and the Transcriptional Regulator FleQ of Legionella pneumophila, Which Are Both Involved in the Regulation Cascade of Flagellar Gene Expression

Sebastian Jacobi,1 Rüdiger Schade,2 and Klaus Heuner1*

Institut für Molekulare Infektionsbiologie, Julius-Maximilians Universität Würzburg, D-97070 Würzburg,1 Institut für Pharmakologie und Toxikologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, 10117 Berlin, Germany2

Received 14 November 2003/ Accepted 13 January 2004

We cloned and analyzed Legionella pneumophila Corby homologs of rpoN (encoding {sigma}54) and fleQ (encoding {sigma}54 activator protein). Two other genes (fleR and pilR) whose products have a {sigma}54 interaction domain were identified in the genome sequence of L. pneumophila. An rpoN mutant strain was nonflagellated and expressed very small amounts of the FlaA (flagellin) protein. Like the rpoN mutant, the fleQ mutant strain of L. pneumophila was also nonflagellated and expressed only small amounts of FlaA protein compared to the amounts expressed by the wild type. In this paper we show that the {sigma}54 factor and the FleQ protein are involved in regulation of flagellar gene operons in L. pneumophila. RpoN and FleQ positively regulate the transcription of FliM and FleN, both of which have a {sigma}54-dependent promoter consensus sequence. However, they seemed to be dispensable for transcription of flaA, fliA, or icmR. Our results confirmed a recently described model of the flagellar gene regulation cascade in L. pneumophila (K. Heuner and M. Steinert, Int. J. Med. Microbiol. 293:133-145, 2003). Flagellar gene regulation was found to be different from that of Enterobacteriaceae but seems to be comparable to that described for Pseudomonas or Vibrio spp.

* Corresponding author. Mailing address: Institut für Molekulare Infektionsbiologie, Julius-Maximilians Universität Würzburg, Röntgenring 11, D-97070 Würzburg, Germany. Phone: 49-931312151. Fax: 49-931312578. E-mail: klaus.heuner{at}mail.uni-wuerzburg.de.

Journal of Bacteriology, May 2004, p. 2540-2547, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2540-2547.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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