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Journal of Bacteriology, May 2004, p. 2699-2707, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2699-2707.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Modulation of DNA Repair and Recombination by the Bacteriophage Orf Function in Escherichia coli K-12

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 2 November 2003/ Accepted 23 January 2004

The orf gene of bacteriophage , fused to a promoter, was placed in the galK locus of Escherichia coli K-12. Orf was found to suppress the recombination deficiency and sensitivity to UV radiation of mutants, in a (recC ptr recB recD)::P_tac gam bet exo pae cI recG background, lacking recF, recO, recR, ruvAB, and ruvC functions. It also suppressed defects of these mutants in establishing replication of a pSC101-related plasmid. Compared to orf, the recA803 allele had only small effects on recF, recO, and recR mutant phenotypes and no effect on a ruvAB mutant. In a fully wild-type background with respect to known recombination and repair functions, orf partially suppressed the UV sensitivity of ruvAB and ruvC mutants.

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