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Journal of Bacteriology, May 2004, p. 2891-2896, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2891-2896.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Origin and Diversity of Alginate Lyases of Families PL-5 and -7 in Sphingomonas sp. Strain A1

Osamu Miyake, Akihito Ochiai, Wataru Hashimoto, and Kousaku Murata*

Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University,Uji, Kyoto 611-0011, Japan

Received 13 November 2003/ Accepted 23 January 2004

Sphingomonas sp. strain A1 has three endotype alginate lyases (A1-I, A1-II [family PL-7], and A1-III [family PL-5]), each of which is encoded by a single gene. In addition to those of these lyases, a gene (the A1-II' gene) showing significant identity with the A1-II gene was present in the bacterial genome and coded for an alginate lyase with broad substrate specificity. Since no expression of A1-II' was observed even in bacterial cells grown on alginate, the A1-II' gene was thought to be a silent gene derived from the A1-II gene, presumably through duplication, modification, and translocation.

* Corresponding author. Mailing address: Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. Phone: 81-774-38-3766. Fax: 81-774-38-3767. E-mail: kmurata{at}kais.kyoto-u.ac.jp.

Journal of Bacteriology, May 2004, p. 2891-2896, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2891-2896.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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