This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ueda, K.
Right arrow Articles by Beppu, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueda, K.
Right arrow Articles by Beppu, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2005, p. 135-142, Vol. 187, No. 1
0021-9193/05/$08.00+0     doi:10.1128/JB.187.1.135-142.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Dual Transcriptional Control of amfTSBA, Which Regulates the Onset of Cellular Differentiation in Streptomyces griseus

Kenji Ueda,* Hideaki Takano, Madoka Nishimoto, Hiromi Inaba, and Teruhiko Beppu

Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, Japan

Received 15 June 2004/ Accepted 16 September 2004

The amf gene cluster encodes a probable secretion system for a peptidic morphogen, AmfS, which induces aerial mycelium formation in Streptomyces griseus. Here we examined the transcriptional control mechanism for the promoter preceding amfT (PamfT) directing the transcription of the amfTSBA operon. High-resolution S1 analysis mapped a transcriptional start point at 31 nucleotides upstream of the translational start codon of amfT. Low-resolution analysis showed that PamfT is developmentally regulated in the wild type and completely abolished in an amfR mutant. The –35 region of PamfT contained the consensus sequence for the binding of BldD, a pleiotropic negative regulator for morphological and physiological development in Streptomyces coelicolor A3(2). The cloned bldD locus of S. griseus showed high sequence similarity to the S. coelicolor counterpart. Transcription of bldD occurred constitutively in both the wild type and an A-factor-deficient mutant of S. griseus, which suggests that the regulatory role of BldD is independent of A-factor. The gel retardation assay revealed that purified BldD and AmfR recombinant proteins specifically bind PamfT. Overproduction of BldD in the wild-type cell conferred a bald phenotype (defective in aerial growth and streptomycin production) and caused marked repression of PamfT activity. An amfT-depleted mutant also showed a bald phenotype but PamfT activity was not affected. Both the bldD-overproducing wild-type strain and the amfT mutant were unable to induce aerial growth of an amfS mutant in a cross-feeding assay, which indicates that these strains are defective in the production of an active AmfS peptide. The results overall suggests that two independent regulators, AmfR and BldD, control PamfT activity via direct binding to determine the transcriptional level of the amf operon responsible for the production and secretion of AmfS peptide, which induces the erection of aerial hyphae in S. griseus.

* Corresponding author. Mailing address: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan. Phone: 81-466-84-3937. Fax: 81-466-84-3935. E-mail: ueda{at}brs.nihon-u.ac.jp.

Journal of Bacteriology, January 2005, p. 135-142, Vol. 187, No. 1
0021-9193/05/$08.00+0     doi:10.1128/JB.187.1.135-142.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hara, H., Ohnishi, Y., Horinouchi, S. (2009). DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus. Microbiology 155: 2197-2210 [Abstract] [Full Text]  
  • Takano, H., Tanaka, T., Beppu, T., Ueda, K. (2008). Identification of sas, a conserved gene cluster involved in the regulation of aerial mycelium formation in Streptomyces griseus. Microbiology 154: 3668-3675 [Abstract] [Full Text]  
  • Ramos, J. L., Martinez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., Tobes, R. (2005). The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69: 326-356 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»