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Journal of Bacteriology, May 2005, p. 3521-3527, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3521-3527.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Linear Double-Stranded DNA of Phage Bam35 Enters Lysogenic Host Cells, but the Late Phage Functions Are Suppressed

Ausra Gaidelyte,1,2 Silja T. Jaatinen,1 Rimantas Daugelavicius,1,2 Jaana K. H. Bamford,1 and Dennis H. Bamford1*

Department of Biological and Environmental Sciences and Institute of Biotechnology, Biocenter 2, P.O. Box 56 (Viikinkaari 5), 00014 University of Helsinki, Finland,1 Department of Biochemistry and Biophysics, Vilnius University, Ciurlionio 21, 03101 Vilnius, Lithuania2

Received 13 December 2004/ Accepted 14 February 2005

Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that encloses the viral DNA. We used electrochemical methods to investigate physiological changes of the lysogenic and nonlysogenic hosts during Bam35 DNA entry and host cell lysis. During viral DNA entry, there was an early temporal decrease of membrane voltage associated with K+ efflux that took place when either lysogenic or nonlysogenic hosts were infected. Approximately 40 min postinfection, a second strong K+ efflux was registered that was proposed to be associated with the insertion of holin molecules into the plasma membrane. This phenomenon occurred only when nonlysogenic cells were infected. Lysogenic hosts rarely were observed entering the lytic cycle as demonstrated by thin-section electron microscopy.

* Corresponding author. Mailing address: Viikki Biocenter, P.O. Box 56 (Viikinkaari 5), FIN-00014, University of Helsinki, Finland. Phone: 358 9 191 59100. Fax: 358 9 191 59098. E-mail: dennis.bamford{at}helsinki.fi.

Journal of Bacteriology, May 2005, p. 3521-3527, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3521-3527.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Gaidelyte, A., Cvirkaite-Krupovic, V., Daugelavicius, R., Bamford, J. K. H., Bamford, D. H. (2006). The Entry Mechanism of Membrane-Containing Phage Bam35 Infecting Bacillus thuringiensis.. J. Bacteriol. 188: 5925-5934 [Abstract] [Full Text]  


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