This Article Full Text Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Qiu, X. Articles by Tiedje, J. M. Search for Related Content PubMed PubMed Citation Articles by Qiu, X. Articles by Tiedje, J. M.

Comparative Analysis of Differentially Expressed Genes in Shewanella oneidensis MR-1 following Exposure to UVC, UVB, and UVA Radiation

Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824,¹ Department of Plant Pathology, Michigan State University, East Lansing, Michigan 48824,² Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831³

Received 23 September 2004/ Accepted 9 February 2005

We previously reported that Shewanella oneidensis MR-1 is highly sensitive to UVC (254 nm), UVB (290 to 320 nm), and UVA (320 to 400 nm). Here we delineated the cellular response of MR-1 to UV radiation damage by analyzing the transcriptional profile during a 1-h recovering period after UVC, UVB, and UVA exposure at a dose that yields about a 20% survival rate. Although the SOS response was observed with all three treatments, the induction was more robust in response to short-wavelength UV radiation (UVB and UVC). Similarly, more prophage-related genes were induced by short-wavelength UV radiation. MR-1 showed an active detoxification mechanism in response to UVA, which included the induction of antioxidant enzymes and iron-sequestering proteins to scavenge reactive oxygen species. In addition, a great number of genes encoding multidrug and heavy metal efflux pumps were induced following UVA irradiation. Our data suggested that activation of prophages appears the major lethal factor in MR-1 following UVC or UVB irradiation, whereas oxidative damage contributes greatly to the high UVA sensitivity in MR-1.

Supplemental material for this article may be found at http://jb.asm.org/.

This article has been cited by other articles:

• Chourey, K., Thompson, M. R., Morrell-Falvey, J., VerBerkmoes, N. C., Brown, S. D., Shah, M., Zhou, J., Doktycz, M., Hettich, R. L., Thompson, D. K. (2006). Global Molecular and Morphological Effects of 24-Hour Chromium(VI) Exposure on Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 72: 6331-6344 [Abstract] [Full Text]  
• Brown, S. D., Thompson, M. R., VerBerkmoes, N. C., Chourey, K., Shah, M., Zhou, J., Hettich, R. L., Thompson, D. K. (2006). Molecular Dynamics of the Shewanella oneidensis Response to Chromate Stress. Mol. Cell. Proteomics 5: 1054-1071 [Abstract] [Full Text]  
• Qiu, X., Daly, M. J., Vasilenko, A., Omelchenko, M. V., Gaidamakova, E. K., Wu, L., Zhou, J., Sundin, G. W., Tiedje, J. M. (2006). Transcriptome Analysis Applied to Survival of Shewanella oneidensis MR-1 Exposed to Ionizing Radiation. J. Bacteriol. 188: 1199-1204 [Abstract] [Full Text]