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The Salmonella Translocated Effector SopA Is Targeted to the Mitochondria of Infected Cells

Abigail N. Layton, Philip J. Brown, and Edouard E. Galyov^*

Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom

Received 6 December 2004/ Accepted 3 February 2005

This study investigates the Salmonella effector protein SopA. We show that in Salmonella enterica serovar Dublin-infected cells, SopA_1-347 fused to two carboxy-terminal hemagglutinin tags partially colocalized with mitochondria. Transfection of eukaryotic cells with a panel of constructs encoding truncated versions of SopA identified that amino acids 100 to 347 were sufficient to target SopA to the mitochondria.

Present address: Nuffield Department of Clinical Lab Sciences, University of Oxford, Level 4 Academic Block, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, United Kingdom.

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