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Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease

Department of Microbiology and Molecular Genetics,¹ Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-4320²

Received 29 November 2004/ Accepted 10 February 2005

Klebsiella aerogenes UreE, a metallochaperone that delivers nickel ions during urease activation, consists of distinct "peptide-binding" and "metal-binding" domains and a His-rich C terminus. Deletion analyses revealed that the metal-binding domain alone is sufficient to facilitate urease activation. This domain was purified and shown to exhibit metal-binding properties similar to those of UreE lacking only the His-rich tail.

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