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Journal of Bacteriology, May 2005, p. 3581-3585, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3581-3585.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease

Scott B. Mulrooney,1 Sarah K. Ward,1 and Robert P. Hausinger1,2*

Department of Microbiology and Molecular Genetics,1 Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-43202

Received 29 November 2004/ Accepted 10 February 2005

Klebsiella aerogenes UreE, a metallochaperone that delivers nickel ions during urease activation, consists of distinct "peptide-binding" and "metal-binding" domains and a His-rich C terminus. Deletion analyses revealed that the metal-binding domain alone is sufficient to facilitate urease activation. This domain was purified and shown to exhibit metal-binding properties similar to those of UreE lacking only the His-rich tail.

* Corresponding author. Mailing address: Microbiology and Molecular Genetics, 6193 Biomedical Physical Sciences, Michigan State University, East Lansing, MI 48824-4320. Phone: (517) 355-6463, ext. 1610. Fax: (517) 353-8957. E-mail: hausinge{at}msu.edu.

Journal of Bacteriology, May 2005, p. 3581-3585, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3581-3585.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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