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Journal of Bacteriology, May 2005, p. 3586-3588, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3586-3588.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bacteriophage Mu Targets the Trinucleotide Sequence CGG

Dipankar Manna,¹ Shuang Deng,¹ Adam M. Breier,^2, and N. Patrick Higgins^1*

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294,¹ Graduate Group in Biophysics, University of California, Berkeley, California 94720²

Received 21 December 2004/ Accepted 31 January 2005

Target specificity for bacteriophage Mu was studied using a new phage derivative that enabled cloning of Mu-host junctions from phage DNA. Insertions distributed throughout the chromosome showed no orientation bias with respect to transcription or replication polarity. Genes with a high frequency of the triplet CGG were preferred targets.

Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.