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Journal of Bacteriology, June 2005, p. 3807-3813, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3807-3813.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of a Turnover Element in Region 2.1 of Escherichia coli {sigma}32 by a Bacterial One-Hybrid Approach

Markus Obrist1,2 and Franz Narberhaus1,2*

Institut für Mikrobiologie, Eidgenössische Technische Hochschule, Zürich, Switzerland,1 Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität Bochum, Bochum, Germany2

Received 22 December 2004/ Accepted 18 February 2005

Induction of the heat shock response in Escherichia coli requires the alternative sigma factor {sigma}32 (RpoH). The cellular concentration of {sigma}32 is controlled by proteolysis involving FtsH, other proteases, and the DnaKJ chaperone system. To identify individual {sigma}32 residues critical for degradation, we used a recently developed bacterial one-hybrid system and screened for stabilized versions of {sigma}32. The five single point mutations that rendered the sigma factor more stable mapped to positions L47, A50, and I54 in region 2.1. Strains expressing the stabilized {sigma}32 variants exhibited elevated transcriptional activity, as determined by a groE-lacZ fusion. Structure calculations predicted that the three mutated residues line up on the same face of an {alpha}-helix in region 2.1, suggesting that they are positioned to interact with proteins of the degradation machinery.

* Corresponding author. Mailing address: Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität Bochum, NDEF 06/783, D-44780 Bochum, Germany. Phone: 49 (234) 32 23100. Fax: 49 (234) 32 14620. E-mail: franz.narberhaus{at}rub.de.

Journal of Bacteriology, June 2005, p. 3807-3813, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3807-3813.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Katz, C., Ron, E. Z. (2008). Dual Role of FtsH in Regulating Lipopolysaccharide Biosynthesis in Escherichia coli. J. Bacteriol. 190: 7117-7122 [Abstract] [Full Text]  
  • Guisbert, E., Yura, T., Rhodius, V. A., Gross, C. A. (2008). Convergence of Molecular, Modeling, and Systems Approaches for an Understanding of the Escherichia coli Heat Shock Response. Microbiol. Mol. Biol. Rev. 72: 545-554 [Abstract] [Full Text]  
  • Yura, T., Guisbert, E., Poritz, M., Lu, C. Z., Campbell, E., Gross, C. A. (2007). Analysis of {sigma}32 mutants defective in chaperone-mediated feedback control reveals unexpected complexity of the heat shock response. Proc. Natl. Acad. Sci. USA 104: 17638-17643 [Abstract] [Full Text]  
  • Obrist, M., Milek, S., Klauck, E., Hengge, R., Narberhaus, F. (2007). Region 2.1 of the Escherichia coli heat-shock sigma factor RpoH ({sigma}32) is necessary but not sufficient for degradation by the FtsH protease. Microbiology 153: 2560-2571 [Abstract] [Full Text]  
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