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Journal of Bacteriology, June 2005, p. 3869-3872, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3869-3872.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Alginate Lyase (AlgL) Activity Is Required for Alginate Biosynthesis in Pseudomonas aeruginosa

Mark T. Albrecht and Neal L. Schiller*

University of California, Riverside, Riverside, California 92521

Received 12 February 2005/ Accepted 1 March 2005

To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algL{Delta}::Gmr mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.

* Corresponding author. Mailing address: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121. Phone: (951) 827-4569. Fax: (951) 827-5504. E-mail: neal.schiller{at}ucr.edu.

Journal of Bacteriology, June 2005, p. 3869-3872, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3869-3872.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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