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Alginate Lyase (AlgL) Activity Is Required for Alginate Biosynthesis in Pseudomonas aeruginosa

University of California, Riverside, Riverside, California 92521

Received 12 February 2005/ Accepted 1 March 2005

To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algL::Gm^r mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.

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