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Journal of Bacteriology, June 2005, p. 3889-3893, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3889-3893.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Gene Encoding L-Methionine {gamma}-Lyase Is Present in Enterobacteriaceae Family Genomes: Identification and Characterization of Citrobacter freundii L-Methionine {gamma}-Lyase

Ilya V. Manukhov,1 Daria V. Mamaeva,2 Sergei M. Rastorguev,1 Nicolai G. Faleev,3 Elena A. Morozova,2 Tatyana V. Demidkina,2,{dagger} and Gennadii B. Zavilgelsky1*,{dagger}

State Research Institute of Genetic and Selection of Industrial Microorganisms, 1st Dorozhnii pr. 1, Moscow 117545, Russia,1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str., 32, Moscow 119991, Russia ,2 Nesmeyanov Institute of Elementoorganic Compounds, Russian Academy of Sciences, Vavilov str., 28, Moscow 119991, Russia3

Received 11 November 2004/ Accepted 21 February 2005

Citrobacter freundii cells produce L-methionine {gamma}-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine {gamma}-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C. freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found.

* Corresponding author. Mailing address: State Research Institute of Genetic and Selection of Industrial Microorganisms, 1st Dorozhnii pr. 1, Moscow 117545, Russia. Phone: (7095) 315 37 29. Fax: (7095) 315 05 01. E-mail: zavilgel{at}genetika.ru.

{dagger} T.V.D. and G.B.Z. contributed equally to this paper.

Journal of Bacteriology, June 2005, p. 3889-3893, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3889-3893.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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