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Journal of Bacteriology, July 2005, p. 4584-4591, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4584-4591.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Host- and Tissue-Specific Pathogenic Traits of Staphylococcus aureus

Willem B. van Leeuwen,1* Damian C. Melles,1 Alwaleed Alaidan,1,2 Mohammed Al-Ahdal,2 Hélène A. M. Boelens,1 Susan V. Snijders,1 Heiman Wertheim,1 Engeline van Duijkeren,3 Justine K. Peeters,4 Peter J. van der Spek,4 Roy Gorkink,5 Guus Simons,5,6 Henri A. Verbrugh,1 and Alex van Belkum1

Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands,1 Department of Molecular Virology and Infectious Diseases, King Faisal Hospital and Research Center, P.O. Box 3354, 11211 Riyadh, Kingdom of Saudi Arabia,2 Veterinary Microbiological Diagnostic Center, Faculty of Veterinary, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands,3 Department of Bioinformatics, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands,4 Department of Microbial Genomics, Keygene B.V., Argo Business Park 90, 6708 PW Wageningen, The Netherlands,5 Pathofinder B.V. Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands6

Received 15 November 2004/ Accepted 27 March 2005

Comparative genomics were used to assess genetic differences between Staphylococcus aureus strains derived from infected animals versus colonized or infected humans. A total of 77 veterinary isolates were genetically characterized by high-throughput amplified fragment length polymorphism (AFLP). Bacterial genotypes were introduced in a large AFLP database containing similar information for 1,056 human S. aureus strains. All S. aureus strains isolated from animals in close contact with humans (e.g., pet animals) were predominantly classified in one of the five main clusters of the AFLP database (cluster I). In essence, mastitis-associated strains from animals were categorized separately (cluster IVa) and cosegregated with bacteremia-associated strains from humans. Distribution of only 2 out of 10 different virulence genes differed across the clusters. The gene encoding the toxic shock syndrome protein (tst) was more often encountered among veterinary strains (P < 0.0001) and even more in the mastitis-related strains (P<0.0001) compared to human isolate results. The gene encoding the collagen binding protein (cna) was rarely detected among invasive human strains. The virulence potential, as indicated by the number of virulence genes per strain, did not differ significantly between the human- and animal-related strains. Our data show that invasive infections in pets and humans are usually due to S. aureus strains with the same genetic background. Mastitis-associated S. aureus isolated in diverse farm animal species form a distinct genetic cluster, characterized by an overrepresentation of the toxic shock syndrome toxin superantigen-encoding gene.

* Corresponding author: Mailing address: Department of Medical Microbiology & Infectious Diseases, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31 10 463 3668. Fax: 31 10 463 3875. E-mail: w.vanleeuwen{at}erasmusmc.nl.

Journal of Bacteriology, July 2005, p. 4584-4591, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4584-4591.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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