This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Waller, L. N.
Right arrow Articles by Shahgholi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waller, L. N.
Right arrow Articles by Shahgholi, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2005, p. 4592-4597, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4592-4597.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of a Second Collagen-Like Glycoprotein Produced by Bacillus anthracis and Demonstration of Associated Spore-Specific Sugars

Lashanda N. Waller,1 Michael J. Stump,1 Karen F. Fox,1 William M. Harley,1 Alvin Fox,1* George C. Stewart,2 and Mona Shahgholi3

Department of Pathology and Microbiology, University of South Carolina School of Medicine, Columbia, South Carolina 29208,1 Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211,2 Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 911255-30003

Received 7 February 2005/ Accepted 28 March 2005

Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A second collagen-like glycoprotein, BclB, was identified in B. anthracis. The protein moiety of this glycoprotein was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB.

* Corresponding author. Mailing address: Department of Pathology and Microbiology, University of South Carolina School of Medicine, Columbia, South Carolina 29208. Phone: 803 733 3288. Fax: 803 733 3192. E-mail: afox{at}med.sc.edu.

Journal of Bacteriology, July 2005, p. 4592-4597, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4592-4597.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Giorno, R., Mallozzi, M., Bozue, J., Moody, K.-S., Slack, A., Qiu, D., Wang, R., Friedlander, A., Welkos, S., Driks, A. (2009). Localization and assembly of proteins comprising the outer structures of the Bacillus anthracis spore. Microbiology 155: 1133-1145 [Abstract] [Full Text]  
  • Caswell, C. C., Barczyk, M., Keene, D. R., Lukomska, E., Gullberg, D. E., Lukomski, S. (2008). Identification of the First Prokaryotic Collagen Sequence Motif That Mediates Binding to Human Collagen Receptors, Integrins {alpha}2{beta}1 and {alpha}11{beta}1. J. Biol. Chem. 283: 36168-36175 [Abstract] [Full Text]  
  • Thompson, B. M., Waller, L. N., Fox, K. F., Fox, A., Stewart, G. C. (2007). The BclB Glycoprotein of Bacillus anthracis Is Involved in Exosporium Integrity. J. Bacteriol. 189: 6704-6713 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»