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Journal of Bacteriology, July 2005, p. 4683-4688, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4683-4688.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Helicobacter pylori CrdRS Two-Component Regulation System (HP1364/HP1365) Is Required for Copper-Mediated Induction of the Copper Resistance Determinant CrdA

Barbara Waidner,1* Klaus Melchers,2,3 Frank Nils Stähler,1 Manfred Kist,1 and Stefan Bereswill1*

Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann-Herder-Straße 11, D-79104 Freiburg, Germany,1 ALTANA Research Institute, 610 Lincoln Street, Waltham, Massachusetts 02451,2 ALTANA Pharma AG, Department of Bioinformatics, Byk-Gulden-Str. 2, D-78467 Konstanz, Germany3

Received 10 February 2005/ Accepted 31 March 2005

Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked copper induction of crdA expression and were copper sensitive. A direct role of CrdR in transcriptional regulation of crdA was confirmed by in vitro binding of CrdR to the crdA upstream region. A 21-nucleotide sequence located near the crdA promoter was shown to be required for CrdR binding.

* Corresponding author. Mailing address for Barbara Waidner: Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann-Herder-Straße 11, D-79104 Freiburg, Germany. Phone: 49-761-203-6539. Fax: 49-761-203-6562. E-mail: Barbara.Waidner{at}uniklinik-freiburg.de. Present address for Stefan Bereswill: Humboldt University, Charité University Medicine Berlin, Charité Campus Mitte, Institute for Microbiology and Hygiene, Dorotheenstraße 96, D-10117 Berlin, Germany. Phone: 49-450-524-006. Fax: 49-30-450-524-904. E-mail: stefan.bereswill{at}charite.de.

Journal of Bacteriology, July 2005, p. 4683-4688, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4683-4688.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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