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The F₁F_o-ATP Synthase of Mycobacterium smegmatis Is Essential for Growth

Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand

Received 26 January 2005/ Accepted 13 April 2005

The F₁F_o-ATP synthase plays an important role in a number of vital cellular processes in plants, animals, and microorganisms. In this study, we constructed a atpD mutant of Mycobacterium smegmatis and demonstrated that atpD encoding the ß subunit of the F₁F_o-ATP synthase is an essential gene in M. smegmatis during growth on nonfermentable and fermentable carbon sources.

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