Journal of Bacteriology, July 2005, p. 5032-5035, Vol. 187, No. 14
0021-9193/05/$08.00+0 doi:10.1128/JB.187.14.5032-5035.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Adenylyl Cyclase Activity of Cya1 from the Cyanobacterium Synechocystis sp. Strain PCC 6803 Is Inhibited by Bicarbonate
Shinji Masuda* and
Taka-aki Ono
Laboratory for Photo-Biology, RIKEN Photodynamics Research Center, The Institute of Physical and Chemical Research, 519-1399 Aramaki, Aoba, Sendai 980-0845, Japan
Received 8 March 2005/
Accepted 11 April 2005
Bicarbonate stimulates the activities of several class III adenylyl cyclases studied to date. However, we show here that bicarbonate decreased Vmax and substrate affinity in Cya1, a major adenylyl cyclase in the cyanobacterium Synechocystis sp. strain PCC 6803. This indicates that manifestation of the bicarbonate responsiveness is specifically modulated in Cya1.
* Corresponding author. Present address: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. Phone: (81) 45-924-5737. Fax: (81) 45-924-5823. E-mail: shmasuda{at}bio.titech.ac.jp.
Journal of Bacteriology, July 2005, p. 5032-5035, Vol. 187, No. 14
0021-9193/05/$08.00+0 doi:10.1128/JB.187.14.5032-5035.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.