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Journal of Bacteriology, August 2005, p. 5267-5277, Vol. 187, No. 15
0021-9193/05/$08.00+0     doi:10.1128/JB.187.15.5267-5277.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cystic Fibrosis Sputum Supports Growth and Cues Key Aspects of Pseudomonas aeruginosa Physiology

Kelli L. Palmer,1,2 Lauren M. Mashburn,1,2 Pradeep K. Singh,3 and Marvin Whiteley1,2*

Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104,1 Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104,2 Division of Pulmonary and Critical Care Medicine, The University of Iowa College of Medicine, Iowa City, Iowa 522403

Received 14 January 2005/ Accepted 2 May 2005

The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum.

* Corresponding author. Mailing address: Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104. Phone: (405) 271-5875. Fax: (405) 271-3874. E-mail: marvin-whiteley{at}ouhsc.edu.

Journal of Bacteriology, August 2005, p. 5267-5277, Vol. 187, No. 15
0021-9193/05/$08.00+0     doi:10.1128/JB.187.15.5267-5277.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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