Journal of Bacteriology, August 2005, p. 5267-5277, Vol. 187, No. 15
0021-9193/05/$08.00+0 doi:10.1128/JB.187.15.5267-5277.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Cystic Fibrosis Sputum Supports Growth and Cues Key Aspects of Pseudomonas aeruginosa Physiology
Kelli L. Palmer,1,2
Lauren M. Mashburn,1,2
Pradeep K. Singh,3 and
Marvin Whiteley1,2*
Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104,1
Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104,2
Division of Pulmonary and Critical Care Medicine, The University of Iowa College of Medicine, Iowa City, Iowa 522403
Received 14 January 2005/
Accepted 2 May 2005
The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum.
* Corresponding author. Mailing address: Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104. Phone: (405) 271-5875. Fax: (405) 271-3874. E-mail: marvin-whiteley{at}ouhsc.edu.
Journal of Bacteriology, August 2005, p. 5267-5277, Vol. 187, No. 15
0021-9193/05/$08.00+0 doi:10.1128/JB.187.15.5267-5277.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Kim, W., Levy, S. B.
(2008). Increased Fitness of Pseudomonas fluorescens Pf0-1 Leucine Auxotrophs in Soil. Appl. Environ. Microbiol.
74: 3644-3651
[Abstract]
[Full Text]
-
Oglesby, A. G., Farrow, J. M. III, Lee, J.-H., Tomaras, A. P., Greenberg, E. P., Pesci, E. C., Vasil, M. L.
(2008). The Influence of Iron on Pseudomonas aeruginosa Physiology: A REGULATORY LINK BETWEEN IRON AND QUORUM SENSING. J. Biol. Chem.
283: 15558-15567
[Abstract]
[Full Text]
-
Lindsey, T. L., Hagins, J. M., Sokol, P. A., Silo-Suh, L. A.
(2008). Virulence determinants from a cystic fibrosis isolate of Pseudomonas aeruginosa include isocitrate lyase. Microbiology
154: 1616-1627
[Abstract]
[Full Text]
-
Yang, L., Haagensen, J. A. J., Jelsbak, L., Johansen, H. K., Sternberg, C., Hoiby, N., Molin, S.
(2008). In Situ Growth Rates and Biofilm Development of Pseudomonas aeruginosa Populations in Chronic Lung Infections. J. Bacteriol.
190: 2767-2776
[Abstract]
[Full Text]
-
del Castillo, T., Duque, E., Ramos, J. L.
(2008). A Set of Activators and Repressors Control Peripheral Glucose Pathways in Pseudomonas putida To Yield a Common Central Intermediate. J. Bacteriol.
190: 2331-2339
[Abstract]
[Full Text]
-
Palmer, K. L., Aye, L. M., Whiteley, M.
(2007). Nutritional Cues Control Pseudomonas aeruginosa Multicellular Behavior in Cystic Fibrosis Sputum. J. Bacteriol.
189: 8079-8087
[Abstract]
[Full Text]
-
Son, M. S., Matthews, W. J. Jr., Kang, Y., Nguyen, D. T., Hoang, T. T.
(2007). In Vivo Evidence of Pseudomonas aeruginosa Nutrient Acquisition and Pathogenesis in the Lungs of Cystic Fibrosis Patients. Infect. Immun.
75: 5313-5324
[Abstract]
[Full Text]
-
Price-Whelan, A., Dietrich, L. E. P., Newman, D. K.
(2007). Pyocyanin Alters Redox Homeostasis and Carbon Flux through Central Metabolic Pathways in Pseudomonas aeruginosa PA14. J. Bacteriol.
189: 6372-6381
[Abstract]
[Full Text]
-
Palmer, K. L., Brown, S. A., Whiteley, M.
(2007). Membrane-Bound Nitrate Reductase Is Required for Anaerobic Growth in Cystic Fibrosis Sputum. J. Bacteriol.
189: 4449-4455
[Abstract]
[Full Text]
-
Yang, L., Barken, K. B., Skindersoe, M. E., Christensen, A. B., Givskov, M., Tolker-Nielsen, T.
(2007). Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa. Microbiology
153: 1318-1328
[Abstract]
[Full Text]
-
Farrow, J. M. III, Pesci, E. C.
(2007). Two Distinct Pathways Supply Anthranilate as a Precursor of the Pseudomonas Quinolone Signal. J. Bacteriol.
189: 3425-3433
[Abstract]
[Full Text]
-
Harrison, F.
(2007). Microbial ecology of the cystic fibrosis lung. Microbiology
153: 917-923
[Abstract]
[Full Text]
-
Marr, A. K., Overhage, J., Bains, M., Hancock, R. E. W.
(2007). The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility. Microbiology
153: 474-482
[Abstract]
[Full Text]
-
Sonawane, A., Jyot, J., During, R., Ramphal, R.
(2006). Neutrophil Elastase, an Innate Immunity Effector Molecule, Represses Flagellin Transcription in Pseudomonas aeruginosa. Infect. Immun.
74: 6682-6689
[Abstract]
[Full Text]
-
McPhee, J. B., Bains, M., Winsor, G., Lewenza, S., Kwasnicka, A., Brazas, M. D., Brinkman, F. S. L., Hancock, R. E. W.
(2006). Contribution of the PhoP-PhoQ and PmrA-PmrB Two-Component Regulatory Systems to Mg2+-Induced Gene Regulation in Pseudomonas aeruginosa. J. Bacteriol.
188: 3995-4006
[Abstract]
[Full Text]
Copyright © 2005 by the American Society for Microbiology. All rights reserved.