The Biotechnology Centre of Oslo and School of Pharmacy, University of Oslo, Oslo, Norway
Received 17 January 2005/ Accepted 12 April 2005
A combination of sequence and structure analysis and reverse transcriptase PCR experiments was used to characterize the group II
introns in the complete genomes of two strains of the pathogen Bacillus cereus. While B. cereus ATCC 14579 harbors a
single intron element in the chromosome, B. cereus ATCC 10987 contains three introns in the chromosome and four in its 208-kb
pBc10987
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subunit of DNA polymerase III. Another striking finding is that the observed 3' splice site of B.c.I4 occurs 56 bp after the predicted end of the intron. This apparently unusual splicing mechanism may be related to structural irregularities in the 3' terminus. Finally, we also show that the intergenic introns of B. cereus ATCC 10987 are transcribed with their upstream genes and do splice in vivo.
* Corresponding author. Mailing address: School of Pharmacy, University of Oslo, PB 1068 Blindern, 0316 Oslo, Norway. Phone: (47) 22 85 63 77. Fax: (47) 22 85 44 42. E-mail: a.b.kolsto{at}admin.uio.no.
Supplemental material for this article may be found at http://jb.asm.org/.
Journal of Bacteriology, August 2005, p. 5437-5451, Vol. 187, No. 15
0021-9193/05/$08.00+0 doi:10.1128/JB.187.15.5437-5451.2005
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