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Journal of Bacteriology, August 2005, p. 5496-5499, Vol. 187, No. 15
0021-9193/05/$08.00+0 doi:10.1128/JB.187.15.5496-5499.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Young-Soon Jang,
Horim Lee, and
Jin-Soo Kim*
ToolGen, Inc., 461-6 Jeonmin-dong, Yuseong-gu, Daejeon, South Korea 305-390
Received 9 March 2005/ Accepted 27 April 2005
We have developed a method with prokaryotic organisms that uses randomized libraries of zinc finger-containing artificial transcription factors to induce phenotypic variations and to identify genes involved in the generation of a specific phenotype of interest. Combining chromatin immunoprecipitation experiments and in silico prediction of target DNA binding sequences for the artificial transcription factors, we identified ubiX, whose down-regulation correlates with the thermotolerance phenotype in Escherichia coli. Our results show that randomized libraries of artificial transcription factors are powerful tools for functional genomic studies.
These authors contributed equally to this work.
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