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Journal of Bacteriology, August 2005, p. 5496-5499, Vol. 187, No. 15
0021-9193/05/$08.00+0     doi:10.1128/JB.187.15.5496-5499.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Phenotypic Alteration and Target Gene Identification Using Combinatorial Libraries of Zinc Finger Proteins in Prokaryotic Cells

Kyung-Soon Park,{dagger} Young-Soon Jang,{dagger} Horim Lee, and Jin-Soo Kim*

ToolGen, Inc., 461-6 Jeonmin-dong, Yuseong-gu, Daejeon, South Korea 305-390

Received 9 March 2005/ Accepted 27 April 2005

We have developed a method with prokaryotic organisms that uses randomized libraries of zinc finger-containing artificial transcription factors to induce phenotypic variations and to identify genes involved in the generation of a specific phenotype of interest. Combining chromatin immunoprecipitation experiments and in silico prediction of target DNA binding sequences for the artificial transcription factors, we identified ubiX, whose down-regulation correlates with the thermotolerance phenotype in Escherichia coli. Our results show that randomized libraries of artificial transcription factors are powerful tools for functional genomic studies.

* Corresponding author. Mailing address: ToolGen, Inc., 461-6 Jeonmin-dong, Yuseong-gu, Daejeon, South Korea 305-390. Phone: 82-42-863-8166. Fax: 82-42-863-3840. E-mail: jsk{at}toolgen.com.

{dagger} These authors contributed equally to this work.

Journal of Bacteriology, August 2005, p. 5496-5499, Vol. 187, No. 15
0021-9193/05/$08.00+0     doi:10.1128/JB.187.15.5496-5499.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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