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Journal of Bacteriology, September 2005, p. 6309-6316, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6309-6316.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Adenylate Cyclase Mutations Rescue the degP Temperature-Sensitive Phenotype and Induce the Sigma E and Cpx Extracytoplasmic Stress Regulons in Escherichia coli

Timothy G. Strozen, Geoffrey R. Langen, and S. Peter Howard^*

Department of Microbiology and Immunology, 107 Wiggins Road, Room A224, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Received 13 April 2005/ Accepted 28 June 2005

Inactivation of the gene encoding the periplasmic protease DegP confers a high-temperature-sensitive phenotype in Escherichia coli. We have previously demonstrated that a degP mutant of E. coli strain CBM (W3110 pldA1) is not temperature sensitive and showed that this was most likely due to constitutive activation of the sigma E and Cpx extracytoplasmic stress regulons in the parent strain. In this study, further characterization of this strain revealed a previously unknown cryptic mutation that rescued the degP temperature-sensitive phenotype by inducing the extracytoplasmic stress regulons. We identified the cryptic mutation as an 11-bp deletion of nucleotides 1884 to 1894 of the adenylate cyclase-encoding cyaA gene (cyaA11). The mechanism in which cyaA11 induces the sigma E and Cpx regulons involves decreased activity of the mutant adenylate cyclase. Addition of exogenous cyclic AMP (cAMP) to the growth medium of a cyaA11 mutant strain that contains a Cpx- and sigma E-inducible degP-lacZ reporter fusion decreased ß-galactosidase expression to levels observed in a cyaA⁺ strain. We also found that a cyaA null mutant displayed even higher levels of extracytoplasmic stress regulon activation compared to a cyaA11 mutant. Thus, we conclude that the lowered concentration of cAMP in cyaA mutants induces both sigma E and Cpx extracytoplasmic stress regulons and thereby rescues the degP temperature-sensitive phenotype.

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* Corresponding author. Mailing address: Department of Microbiology and Immunology, 107 Wiggins Road, Room A224, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada. Phone: (306) 966-2548. Fax: (306) 966-4311. E-mail: peter.howard{at}usask.ca.

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Journal of Bacteriology, September 2005, p. 6309-6316, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6309-6316.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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