This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mostowy, S.
Right arrow Articles by Behr, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mostowy, S.
Right arrow Articles by Behr, M. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2005, p. 6386-6395, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6386-6395.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Revisiting the Evolution of Mycobacterium bovis

Serge Mostowy,1 Jackie Inwald,2 Steve Gordon,2 Carlos Martin,3 Rob Warren,4 Kristin Kremer,5 Debby Cousins,6 and Marcel A. Behr1*

McGill University Health Centre, Montreal, Quebec H3G 1A4, Canada,1 Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom,2 Grupo de Genetica de Micobacterias, Departamento de Microbiologia Medicina Preventiva y Salud Publica, Universidad de Zaragoza, Spain,3 MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, University of Stellenbosch, P.O. Box 19063, Tygerberg 7505, South Africa,4 Mycobacteria Reference Unit, Diagnostic Laboratory of Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands,5 Australian Reference Laboratory for Bovine Tuberculosis, Department of Agriculture, South Perth 6151, Australia6

Received 25 April 2005/ Accepted 27 June 2005

Though careful consideration has been placed towards genetic characterization of tubercle bacillus isolates causing disease in humans, those causing disease predominantly among wild and domesticated mammals have received less attention. In contrast to Mycobacterium tuberculosis, whose host range is largely specific to humans, M. bovis and "M bovis-like" organisms infect a broad range of animal species beyond their most prominent host in cattle. To determine whether strains of variable genomic content are associated with distinct distributions of disease, the DNA contents of M. bovis or M. bovis-like isolates from a variety of hosts were investigated via Affymetrix GeneChip. Consistent with previous genomic analysis of the M. tuberculosis complex (MTC), large sequence polymorphisms of putative diagnostic and biological consequence were able to unambiguously distinguish interrogated isolates. The distribution of deleted regions indicates organisms genomically removed from M. bovis and also points to structured genomic variability within M. bovis. Certain genomic profiles spanned a variety of hosts but were clustered by geography, while others associated primarily with host type. In contrast to the prevailing assumption that M. bovis has broad host capacity, genomic profiles suggest that distinct MTC lineages differentially infect a variety of mammals. From this, a phylogenetic stratification of genotypes offers a predictive framework upon which to base future genetic and phenotypic studies of the MTC.

* Corresponding author. Mailing address: Division of Infectious Diseases and Medical Microbiology, A5-156, Montreal General Hospital, 1650 Cedar Avenue, Montreal QC H3G 1A4, Canada. Phone: 514 934 1934 42815. Fax: 514 934 8423. E-mail: marcel.behr{at}mcgill.ca.

Journal of Bacteriology, September 2005, p. 6386-6395, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6386-6395.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kaser, M., Gutmann, O., Hauser, J., Stinear, T., Cole, S., Yeboah-Manu, D., Dernick, G., Certa, U., Pluschke, G. (2009). Lack of Insertional-Deletional Polymorphism in a Collection of Mycobacterium ulcerans Isolates from Ghanaian Buruli Ulcer Patients. J. Clin. Microbiol. 47: 3640-3646 [Abstract] [Full Text]  
  • Muller, B., Hilty, M., Berg, S., Garcia-Pelayo, M. C., Dale, J., Boschiroli, M. L., Cadmus, S., Ngandolo, B. N. R., Godreuil, S., Diguimbaye-Djaibe, C., Kazwala, R., Bonfoh, B., Njanpop-Lafourcade, B. M., Sahraoui, N., Guetarni, D., Aseffa, A., Mekonnen, M. H., Razanamparany, V. R., Ramarokoto, H., Djonne, B., Oloya, J., Machado, A., Mucavele, C., Skjerve, E., Portaels, F., Rigouts, L., Michel, A., Muller, A., Kallenius, G., van Helden, P. D., Hewinson, R. G., Zinsstag, J., Gordon, S. V., Smith, N. H. (2009). African 1, an Epidemiologically Important Clonal Complex of Mycobacterium bovis Dominant in Mali, Nigeria, Cameroon, and Chad. J. Bacteriol. 191: 1951-1960 [Abstract] [Full Text]  
  • Wheeler, P. R., Brosch, R., Coldham, N. G., Inwald, J. K., Hewinson, R. G., Gordon, S. V. (2008). Functional analysis of a clonal deletion in an epidemic strain of Mycobacterium bovis reveals a role in lipid metabolism. Microbiology 154: 3731-3742 [Abstract] [Full Text]  
  • Golby, P., Hatch, K. A., Bacon, J., Cooney, R., Riley, P., Allnutt, J., Hinds, J., Nunez, J., Marsh, P. D., Hewinson, R. G., Gordon, S. V. (2007). Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology 153: 3323-3336 [Abstract] [Full Text]  
  • Flores, L., Van, T., Narayanan, S., DeRiemer, K., Kato-Maeda, M., Gagneux, S. (2007). Large Sequence Polymorphisms Classify Mycobacterium tuberculosis Strains with Ancestral Spoligotyping Patterns. J. Clin. Microbiol. 45: 3393-3395 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiol. Mol. Biol. Rev. 71: 495-548 [Abstract] [Full Text]  
  • Vishnoi, A., Roy, R., Bhattacharya, A. (2007). Comparative analysis of bacterial genomes: identification of divergent regions in mycobacterial strains using an anchor-based approach. Nucleic Acids Res 35: 3654-3667 [Abstract] [Full Text]  
  • Turenne, C. Y., Wallace, R. Jr., Behr, M. A. (2007). Mycobacterium avium in the Postgenomic Era. Clin. Microbiol. Rev. 20: 205-229 [Abstract] [Full Text]  
  • Taylor, G. M., Murphy, E., Hopkins, R., Rutland, P., Chistov, Y. (2007). First report of Mycobacterium bovis DNA in human remains from the Iron Age. Microbiology 153: 1243-1249 [Abstract] [Full Text]  
  • Brosch, R., Gordon, S. V., Garnier, T., Eiglmeier, K., Frigui, W., Valenti, P., Dos Santos, S., Duthoy, S., Lacroix, C., Garcia-Pelayo, C., Inwald, J. K., Golby, P., Garcia, J. N., Hewinson, R. G., Behr, M. A., Quail, M. A., Churcher, C., Barrell, B. G., Parkhill, J., Cole, S. T. (2007). Genome plasticity of BCG and impact on vaccine efficacy. Proc. Natl. Acad. Sci. USA 104: 5596-5601 [Abstract] [Full Text]  
  • Huard, R. C., Fabre, M., de Haas, P., Claudio Oliveira Lazzarini, L., van Soolingen, D., Cousins, D., Ho, J. L. (2006). Novel Genetic Polymorphisms That Further Delineate the Phylogeny of the Mycobacterium tuberculosis Complex.. J. Bacteriol. 188: 4271-4287 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»